Department of Physiology and Pathophysiology, Shanghai Medical College Fudan University, Shanghai 200032, PR China.
Lipids Health Dis. 2010 Mar 5;9:22. doi: 10.1186/1476-511X-9-22.
The master clock within the hypothalamic suprachiasmatic nucleus (SCN) synchronizing clocks in peripheral tissues is entrained by the environmental condition, such as the light-dark (LD) cycle. The mechanisms of circadian clockwork are similar in both SCN and peripheral tissues. The aim of the present work was to observe the profiles of clock genes expression in mouse central and peripheral tissues within postnatal day 5 (P5). The daily expression of four clock genes mRNA (Bmal1, Per2, Cry1 and Rev-erb alpha) in mouse SCN and heart was measured at P1, P3 and P5 by real-time PCR.
All the studied mice clock genes began to express in a circadian rhythms manner in heart and SCN at P3 and P5 respectively. Interestingly, the daily rhythmic phase of some clock genes shifted during the postnatal days. Moreover, the expressions of clock genes in heart were not synchronized with those in SCN until at P5.
The data showed the gradual development of clock genes in SCN and a peripheral tissue, and suggested that development of clock genes differed between in the SCN and the heart. Judging from the mRNA expression, it was possible that the central clock synchronized the peripheral clock as early as P5.
下丘脑视交叉上核（SCN）中的主钟通过环境条件（如明暗循环）来同步外周组织中的时钟。SCN 和外周组织中的生物钟机制相似。本研究旨在观察新生后第 5 天（P5）小鼠中枢和外周组织中时钟基因表达的特征。通过实时 PCR 测量 P1、P3 和 P5 时，在小鼠 SCN 和心脏中测量了四个时钟基因 mRNA（Bmal1、Per2、Cry1 和 Rev-erb alpha）的每日表达。
所有研究的小鼠时钟基因在 P3 和 P5 时分别在心脏和 SCN 中以昼夜节律方式开始表达。有趣的是，一些时钟基因的每日节律相位在出生后几天内发生了变化。此外，心脏中的时钟基因表达与 SCN 中的表达不同步，直到 P5 时才同步。
数据显示 SCN 和外周组织中时钟基因的逐渐发育，并表明 SCN 和心脏中的时钟基因发育不同。从 mRNA 表达来看，中央时钟可能早在 P5 时就已经同步了外周时钟。
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