Department of Microbiology and Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, PR China.
Cell Immunol. 2010;262(1):11-7. doi: 10.1016/j.cellimm.2009.08.004. Epub 2009 Aug 15.
beta-1,4-galactosyltransferase-I (beta-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of beta-1,4-GalT-I were expressed in human CD4(+) T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of beta-1,4-GalT-I was increased in CD4(+) T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2+IL-12, but decreased after stimulation with IL-2+IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4(+) T cells was significantly increased upon cytokine stimulation, and was inhibited by alpha-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long beta-1,4-GalT-I. Collectively, the data suggest that beta-1,4-GalT-I plays a role in the cellular adhesion of CD4(+) T cells.
β-1,4-半乳糖基转移酶-I（β-1,4-GalT-I）有两种同工酶，它们仅在细胞质结构域的长度上有所不同。在本研究中，我们发现β-1,4-GalT-I 的长型和短型同工酶均在人 CD4+T 淋巴细胞中表达，并定位于细胞质和质膜上。白细胞介素（IL）-2 刺激后，CD4+T 细胞中β-1,4-GalT-I 的表达水平增加，而在 IL-2+IL-12 刺激后进一步增加，但与单独 IL-2 刺激相比，IL-2+IL-4 刺激后则降低。我们还证明，细胞因子刺激后 CD4+T 细胞的细胞黏附显著增加，并且被α-乳白蛋白抑制，表明黏附的增加与长型β-1,4-GalT-I 的表达和活性呈正相关。总之，这些数据表明β-1,4-GalT-I 在 CD4+T 细胞的细胞黏附中发挥作用。
