National Bioenergy Center, National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA.
J Chromatogr A. 2010 Apr 2;1217(14):2082-7. doi: 10.1016/j.chroma.2010.01.061. Epub 2010 Feb 4.
A simple, precise, and accurate 10-min high pressure liquid chromatography (HPLC) method was developed and validated for the analysis of organic acids, alcohols, and furans from processing biomass into renewable fuels. The method uses an H(+) form cation-exchange resin stationary phase that has a five-fold shorter analysis time versus that in the traditional method. The new method was used for the analysis of acetic acid, ethanol, 5-hydroxymethyl furfural, and furfural. Results were compared with a legacy method that has historically has been used to analyze the same compounds but with a 55 min run time. Linearity was acceptable on the new method with r(2)>0.999 for all compounds using refractive index detection. Limits of detection were between 0.003 and 0.03 g/L and limits of quantification were between 0.1 and 0.01 g/L. The relative standard deviations for precision were less than 0.4% and recoveries ranged from 92% to 114% for all compounds.
建立并验证了一种简单、精确、准确的 10 分钟高压液相色谱（HPLC）方法，用于分析从生物质加工成可再生燃料过程中的有机酸、醇和呋喃。该方法使用 H（+）形式的阳离子交换树脂固定相，与传统方法相比，分析时间缩短了五倍。该新方法用于分析乙酸、乙醇、5-羟甲基糠醛和糠醛。结果与历史上用于分析相同化合物但运行时间为 55 分钟的传统方法进行了比较。使用折射指数检测，新方法的线性度良好，所有化合物的 r（2）均大于 0.999。在新方法中，所有化合物的检测限在 0.003 至 0.03 g/L 之间，定量限在 0.1 至 0.01 g/L 之间。对于所有化合物，精密度的相对标准偏差均小于 0.4%，回收率在 92%至 114%之间。
