Spano Nadia, Ciulu Marco, Floris Ignazio, Panzanelli Angelo, Pilo Maria I, Piu Paola C, Salis Severyn, Sanna Gavino
Università degli Studi di Sassari, Dipartimento di Chimica, Sassari, Italy.
Talanta. 2009 Apr 15;78(1):310-4. doi: 10.1016/j.talanta.2008.11.015. Epub 2008 Nov 24.
In this study 5-hydroxymethyl-2-furaldehyde (HMF), 2-furaldehyde, 3-furaldehyde, 2-furoic acid and 3-furoic acid are contemporarily determined in honey using a swift and direct RP-HPLC approach. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and reproducibility), linearity and accuracy (by recovery tests); the acceptability of the precision and accuracy results was positively verified using Horwitz's model and AOAC guidelines, respectively. The method was tested on 18 honey samples of different ages, and botanical and geographical origin. HMF and 2-furaldehyde correlated highly with the age of the samples, whereas no correlation was observed with regards to 2-furaldehyde and 2-furoic acid. Hypotheses relating to the formation of minority furanic compounds are also proposed.
在本研究中，采用快速直接的反相高效液相色谱法同时测定蜂蜜中的5-羟甲基-2-糠醛（HMF）、2-糠醛、3-糠醛、2-糠酸和3-糠酸。按照检测限和定量限、精密度（通过重复性和再现性）、线性和准确度（通过回收率试验）进行验证方案；分别使用霍维茨模型和AOAC指南对精密度和准确度结果的可接受性进行了肯定验证。该方法在18个不同年龄、植物来源和地理来源的蜂蜜样品上进行了测试。HMF和2-糠醛与样品年龄高度相关，而2-糠醛和2-糠酸之间未观察到相关性。还提出了与少量呋喃类化合物形成有关的假设。
