Department of Pediatric Dentistry, Iwate Medical University, 19-1 Uchimaru, Morioka, Japan.
Dent Mater. 2010 Jun;26(6):e181-7. doi: 10.1016/j.dental.2009.10.006. Epub 2010 Mar 3.
The purpose of the present investigation was to trace the fate of bisphenol A injected into pregnant mice, focusing on its potential accumulation in the fetus and the brain, critical targets of hormonal chemicals, using whole-body autoradiography.
Pregnant mice were injected intraperitoneally with 0.46MBq of (14)C-BPA and then killed at 1h or 1, 3, or 5 days after injection. Sections for autoradiography were prepared in a cryomicrotome and the exposed imaging plate was processed using a fluorescent/radioisotope image analyzer.
Intraperitoneally injected (14)C-BPA was distributed throughout the body, including the fetus and the brain, within 1h. Radioactivity faded gradually from the whole body by the fifth day, and no accumulation in any specific organ was found. However, although (14)C was detected in the fetuses immediately after injection, the transfer of BPA from mother to newborn was not observed.
The routes of rapid BPA discharge were confirmed, and BPA neither accumulated in the body nor was it transferred to newborn mice. No evidence was observed to suggest the existence of a blood-placenta or blood-brain barrier for BPA. This information should be taken into consideration when assessing the risks of using dental materials that contain BPA.
本研究旨在利用整体放射自显影技术，追踪注射到怀孕小鼠体内的双酚 A 的去向，重点研究其在胎儿和大脑（激素化学物质的关键靶标）中的潜在蓄积情况。
将怀孕小鼠经腹腔注射 0.46MBq 的 (14)C-BPA，然后在注射后 1h 或 1、3 或 5 天处死。在 cryomicrotome 中制备用于放射自显影的切片，并用荧光/放射性同位素图像分析仪处理暴露的成像板。
在 1h 内，注射到腹腔内的 (14)C-BPA 分布于全身，包括胎儿和大脑。第五天，放射性逐渐从全身消失，未发现任何特定器官的蓄积。然而，尽管在注射后立即在胎儿中检测到 (14)C，但未观察到 BPA 从母体转移到新生小鼠。
证实了 BPA 的快速排泄途径，BPA 既未在体内蓄积，也未转移到新生小鼠体内。没有证据表明存在 BPA 的血胎盘或血脑屏障。在评估含有 BPA 的牙科材料的风险时，应考虑到这些信息。
