Zhang Feng, Yang Gou-li, He Fu-ming, Zhang Li-juan, Zhao Shi-fang
Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
J Oral Maxillofac Surg. 2010 May;68(5):1131-9. doi: 10.1016/j.joms.2009.12.027. Epub 2010 Mar 3.
The purpose of this study was to investigate the effect of surface chemistry of a sandblasted and acid-etched implant (with and without titanium hydride [TiH(2)]) on cell attachment, proliferation, and differentiation of preosteoblasts (MC3T3-E1).
Sandblasted and dual acid-etched titanium discs comprised the test group, whereas sandblasted, acid-etched, and heat-treated discs comprised the control group. Both groups' discs were sent for surface characterization. MC3T3-E1 cells were cultured on these 2 groups' discs, and then cell attachment, cell proliferation, and cell differentiation were analyzed.
Scanning electron microscope analysis showed that the titanium discs in the 2 groups shared the same surface topography; however, x-ray diffraction examination showed that the TiH(2) diffractions only appeared in the test group. Cell attachment and cell proliferation were much better in the test group than in the control group at all time points investigated (P < .05). The expressions of alkaline phosphatase and osteocalcin were significantly higher in the test group than in the control group for both protein and transcription level at every time point (P < .05 or P < .01).
These results suggested that surface chemistry played a significant role in cell response to the sandblasted and acid-etched surface and the presence of TiH(2) might promote the attachment, proliferation, and differentiation of preosteoblasts.
本研究旨在探讨喷砂及酸蚀种植体（有或无氢化钛[TiH₂]）的表面化学性质对前成骨细胞（MC3T3-E1）的细胞黏附、增殖及分化的影响。
喷砂及双重酸蚀钛盘构成试验组，而喷砂、酸蚀及热处理钛盘构成对照组。两组钛盘均送去进行表面特性分析。将MC3T3-E1细胞培养在这两组钛盘上，然后分析细胞黏附、细胞增殖及细胞分化情况。
扫描电子显微镜分析显示，两组钛盘具有相同的表面形貌；然而，X射线衍射检查显示，TiH₂衍射仅出现在试验组。在所有研究的时间点，试验组的细胞黏附和细胞增殖均明显优于对照组（P < 0.05）。在每个时间点，试验组碱性磷酸酶和骨钙素在蛋白质及转录水平的表达均显著高于对照组（P < 0.05或P < 0.01）。
这些结果表明，表面化学性质在细胞对喷砂及酸蚀表面的反应中起重要作用，且TiH₂的存在可能促进前成骨细胞的黏附、增殖及分化。
