Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey.
Clin Hemorheol Microcirc. 2010;44(2):87-96. doi: 10.3233/CH-2010-1254.
This study aimed to investigate the short term effects of carbon monoxide (CO) poisoning and three kinds of poisoning treatments; namely room air, normobaric and hyperbaric oxygen on hemorheological parameters such as red blood cell (RBC) deformability, aggregation, blood and plasma viscosity. 43 Wistar rats were divided into 5 groups. Poisoning was induced by exposure to 4000 ppm CO (1 h). The poisoning protocol was followed by 3 types of treatments; room air, normobaric 100% oxygen and hyperbaric oxygen for 1 h. RBC deformability and aggregation were determined using an ektacytometer (LORCA) and a cone-plate rotational viscometer was used for the viscosity measurements. RBC deformability of CO poisoned rats were found to be elevated and the treatments applied, caused decrement of this parameter. A no significant increment tendency was found in erythrocyte aggregation after CO exposure. Although room air and hyperbaric oxygen treatments caused further significant elevations in the amplitude of aggregation, normobaric oxygen therapy induced decrement in this parameter towards control levels. No significant alterations were observed in viscosity values among the groups. The results of this study demonstrate normobaric oxygen therapy as a better choice of treatment after CO poisoning in hemorheological point of view.
本研究旨在探讨一氧化碳（CO）中毒及其三种中毒治疗方法（即常压空气、常压和高压氧）对红细胞（RBC）变形性、聚集性、血液和血浆粘度等血液流变学参数的短期影响。将 43 只 Wistar 大鼠分为 5 组。通过暴露于 4000 ppm CO（1 小时）来诱导中毒。中毒方案后进行三种治疗方法；常压空气、常压 100%氧和高压氧 1 小时。使用 ektacytometer（LORCA）测定 RBC 变形性和聚集性，使用锥板旋转粘度计测量粘度。发现 CO 中毒大鼠的 RBC 变形性升高，应用的治疗方法导致该参数降低。CO 暴露后红细胞聚集的幅度没有明显的增加趋势。尽管常压空气和高压氧治疗导致聚集幅度进一步显著升高，但常压氧疗使该参数向对照水平降低。各组之间的粘度值无明显变化。本研究结果表明，从血液流变学的角度来看，高压氧治疗是 CO 中毒后更好的治疗选择。
