Dalzell P, Miles L G, Isberg S R, Glenn T C, King C, Murtagh V, Moran C
South Eastern Area Laboratory Services, Randwick, NSW, Australia.
Cytogenet Genome Res. 2009;127(2-4):204-12. doi: 10.1159/000293286. Epub 2010 Mar 4.
Basic cytogenetic data, such as diploid number and general chromosome morphology, are available for many reptilian species. Here we present a detailed cytogenetic examination of the saltwater crocodile (Crocodylus porosus) karyotype, including the creation of the first fully annotated G-band standard ideogram for any crocodilian species. The C. porosus karyotype contains macrochromosomes and has a diploid number of 34. This study presents a detailed description of each chromosome, permitting unambiguous chromosome identification. The fully annotated standardized C. porosus ideogram provides the backbone to a standard nomenclature system which can be used to accurately identify specific band locations. Seven microsatellite containing fosmid clones were fluorescently labeled and used as fluorescent in situ hybridization (FISH) probes for physical localization. Chromosome locations for each of these FISH probes were successfully assigned, demonstrating the utility of the fully annotated ideogram for genome mapping.
许多爬行动物物种都有基本的细胞遗传学数据，如二倍体数目和一般染色体形态。在此，我们对咸水鳄（湾鳄）的核型进行了详细的细胞遗传学检查，包括为任何鳄类物种创建首个完全注释的G带标准 ideogram。湾鳄的核型包含大染色体，二倍体数目为34。本研究对每条染色体进行了详细描述，从而能够明确无误地识别染色体。完全注释的标准化湾鳄ideogram为标准命名系统提供了基础，该系统可用于准确识别特定带的位置。七个含有微卫星的fosmid克隆被荧光标记，并用作荧光原位杂交（FISH）探针进行物理定位。这些FISH探针中的每一个都成功地确定了染色体位置，证明了完全注释的ideogram在基因组图谱绘制中的实用性。
