Cancer Biology Research Center, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China.
Oncol Rep. 2010 Apr;23(4):1063-8.
The aim of this study was to determine whether the universal overexpression of hiwi is associated with cancer angiogenesis. The difference of mRNA expression in blood vessels derived from DLBCL and RLNH tissues was detected, and hiwi was overexpressed in tumor vessels of lymphoma. The hiwi expression in cell lines and tissues was measured by using RT-PCR and immuno-staining, as well as the Ang-2 and Tie-2 expressions in tissues. Hiwi was significantly expressed in cancer cell lines Hela, MCF-7, MDA-MB-231, MDA-MB-435s, A2780, 111 and 113. Compared with that of chronic cervicitis (CC), hyperplasia of mammary glands (HMG), ovarian benign lesions (OBL) and endometrium benign lesions (EBL), the expression of hiwi, Ang-2 and Tie-2 was increased significantly in uterine cervical cancer (UCC), breast carcinoma (BC), ovarian cancer (OC) and endometrial cancer (EC) (P<0.01). Additionally, the hiwi expression was positively correlated with Ang-2 in UCC, BC and OC, and Tie-2 in OC. These results suggest that hiwi plays an important role in the oncogenesis and is a potential target for cancer angiogenesis.
本研究旨在探讨 hiwi 的普遍过表达是否与癌症血管生成有关。检测来源于 DLBCL 和 RLNH 组织的血管中 mRNA 的表达差异，并在淋巴瘤肿瘤血管中过表达 hiwi。通过 RT-PCR 和免疫染色检测细胞系和组织中的 hiwi 表达，以及组织中 Ang-2 和 Tie-2 的表达。在癌细胞系 Hela、MCF-7、MDA-MB-231、MDA-MB-435s、A2780、111 和 113 中，hiwi 明显表达。与慢性宫颈炎（CC）、乳腺增生（HMG）、卵巢良性病变（OBL）和子宫内膜良性病变（EBL）相比，子宫颈癌（UCC）、乳腺癌（BC）、卵巢癌（OC）和子宫内膜癌（EC）中 hiwi、Ang-2 和 Tie-2 的表达明显增加（P<0.01）。此外，hiwi 在 UCC、BC 和 OC 中与 Ang-2 呈正相关，在 OC 中与 Tie-2 呈正相关。这些结果表明，hiwi 在肿瘤发生中发挥重要作用，是癌症血管生成的潜在靶点。
