Japan International Research Center for Agricultural Sciences, Ohwashi, Tsukuba, Ibaraki, Japan.
Theor Appl Genet. 2010 Jul;121(2):229-36. doi: 10.1007/s00122-010-1304-y. Epub 2010 Mar 5.
Salt-affected soils are generally classified into two main categories, sodic (alkaline) and saline. Our previous studies showed that the wild soybean accession JWS156-1 (Glycine soja) from the Kinki area of Japan was tolerant to NaCl salt, and the quantitative trait locus (QTL) for NaCl salt tolerance was located on soybean linkage group N (chromosome 3). Further investigation revealed that the wild soybean accession JWS156-1 also had a higher tolerance to alkaline salt stress. In the present study, an F(6) recombinant inbred line mapping population (n = 112) and an F(2) population (n = 149) derived from crosses between a cultivated soybean cultivar Jackson and JWS156-1 were used to identify QTL for alkaline salt tolerance in soybean. Evaluation of soybean alkaline salt tolerance was carried out based on salt tolerance rating (STR) and leaf chlorophyll content (SPAD value) after treatment with 180 mM NaHCO(3) for about 3 weeks under greenhouse conditions. In both populations, a significant QTL for alkaline salt tolerance was detected on the molecular linkage group D2 (chromosome 17), which accounted for 50.2 and 13.0% of the total variation for STR in the F(6) and the F(2) populations, respectively. The wild soybean contributed to the tolerance allele in the progenies. Our results suggest that QTL for alkaline salt tolerance is different from the QTL for NaCl salt tolerance found previously in this wild soybean genotype. The DNA markers closely associated with the QTLs might be useful for marker-assisted selection to pyramid tolerance genes in soybean for both alkaline and saline stresses.
受盐影响的土壤通常分为两类，即苏打（碱性）和盐渍土。我们之前的研究表明，来自日本近畿地区的野生大豆品种 JWS156-1（Glycine soja）对 NaCl 盐具有耐受性，耐 NaCl 盐的数量性状基因座（QTL）位于大豆连锁群 N（染色体 3）上。进一步的研究表明，野生大豆品种 JWS156-1 对碱性盐胁迫也有更高的耐受性。在本研究中，利用一个来自栽培大豆品种 Jackson 和 JWS156-1 的杂交 F(6)重组自交系（n = 112）和 F(2)群体（n = 149），鉴定了大豆耐碱性盐的 QTL。在温室条件下，用 180mM NaHCO(3)处理约 3 周后，基于耐盐评分（STR）和叶片叶绿素含量（SPAD 值）对大豆的碱性盐耐受性进行评估。在两个群体中，均在分子连锁群 D2（染色体 17）上检测到一个显著的耐碱性盐 QTL，该 QTL 分别占 F(6)和 F(2)群体 STR 总变异的 50.2%和 13.0%。野生大豆为后代提供了耐盐等位基因。我们的研究结果表明，耐碱性盐的 QTL 与之前在该野生大豆基因型中发现的耐 NaCl 盐的 QTL 不同。与 QTL 紧密相关的 DNA 标记可能有助于大豆耐碱性和盐胁迫的基因聚合的标记辅助选择。
