Shi XiaoLei, Yan Long, Yang ChunYan, Yan WeiWen, Moseley David Octor, Wang Tao, Liu BingQiang, Di Rui, Chen PengYin, Zhang MengChen
Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Shijiazhuang Branch Center of National Center for Soybean Improvement/Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture/Key Laboratory of Crop Genetics and Breeding, Shijiazhuang, 050035, China.
Department of Crop, Soil and Environment Sciences, University of Arkansas, Fayetteville, AR, 72701, USA.
BMC Res Notes. 2018 Feb 5;11(1):95. doi: 10.1186/s13104-018-3202-3.
Identification of the quantitative trait locus (QTL) underlying salt tolerance is a prerequisite for marker-assisted selection in the salt-tolerant breeding process.
In this study, the recombinant inbred lines derived from the salt-tolerant elite soybean cultivar 'Jidou 12' and the salt-sensitive elite cultivar 'Ji NF 58' were used to identify the QTL associated with salt tolerance, using both salt tolerance rating (STR) and leaf chlorophyll content (SPAD) as indicators.
A major salt-tolerant QTL, which was flanked by SSR markers GMABAB and Barcsoyssr_03_1421 on chromosome 3, was identified based on single-marker regression, single trait composite interval mapping, and multiple interval mapping analysis. For STR, the LOD ranged from 19.8 to 20.1; R ranged from 44.3 to 44.7%; and the additive effect ranged from 0.876 to 0.885 among the three mapping methods. For SPAD, the LOD ranged from 10.6 to 11.0; R ranged from 27.0 to 27.6%; and the additive effect ranged from 1.634 to 1.679 among the three mapping methods.
In this study, a major QTL conditioning salt tolerance on chromosome 3 was identified. The DNA markers closely associated with the QTLs might be useful in marker-assisted selection for soybean salt tolerance improvement in Huanghuaihai, China.
鉴定耐盐性相关的数量性状位点（QTL）是耐盐育种过程中标记辅助选择的前提条件。
本研究中，以耐盐优良大豆品种‘冀豆12’和盐敏感优良品种‘冀NF58’衍生的重组自交系为材料，以耐盐等级（STR）和叶片叶绿素含量（SPAD）为指标，鉴定与耐盐性相关的QTL。
基于单标记回归、单性状复合区间作图和多区间作图分析，在3号染色体上鉴定出一个主要的耐盐QTL，其两侧为SSR标记GMABAB和Barcsoyssr_03_1421。对于STR，三种作图方法的LOD值在19.8至20.1之间；R值在44.3至44.7%之间；加性效应在0.876至0.885之间。对于SPAD，三种作图方法的LOD值在10.6至11.0之间；R值在27.0至27.6%之间；加性效应在1.634至1.679之间。
本研究在3号染色体上鉴定出一个控制耐盐性的主要QTL。与这些QTL紧密连锁的DNA标记可能有助于中国黄淮海地区大豆耐盐性改良的标记辅助选择。
