Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre, Lange Kleiweg 139, 2288GJ, Rijswijk, The Netherlands.
Immunogenetics. 2010 May;62(5):295-306. doi: 10.1007/s00251-010-0434-3. Epub 2010 Mar 5.
To further refine and improve biomedical research in rhesus macaques, it is necessary to increase our knowledge concerning both the degree of allelic variation (polymorphism) and diversity (gene copy number variation) in the killer cell immunoglobulin-like receptor (KIR) gene cluster. Pedigreed animals in particular should be studied, as segregation data will provide clues to the linkage of particular KIR genes/alleles segregating on a haplotype and to its gene content as well. A dual strategy allowed us to screen the presence and absence of genes and the corresponding transcripts, as well as to track differences in transcription levels. On the basis of this approach, 14 diverse KIR haplotypes have been described. These haplotypes consist of multiple inhibitory and activating Mamu-KIR genes, and any gene present on one haplotype may be absent on another. This suggests that the cost of accelerated evolution by recombination may be the loss of certain framework genes on a haplotype.
为了进一步完善和提高恒河猴的医学研究，我们有必要增加对杀伤细胞免疫球蛋白样受体（KIR）基因簇等位基因变异（多态性）和多样性（基因拷贝数变异）程度的了解。特别是应该研究有谱系的动物，因为分离数据将为特定 KIR 基因/等位基因在单倍型上的分离以及其基因组成提供线索。双重策略使我们能够筛选基因和相应转录本的存在和缺失，以及跟踪转录水平的差异。在此基础上，已经描述了 14 种不同的 KIR 单倍型。这些单倍型由多个抑制和激活的 Mamu-KIR 基因组成，一个单倍型上存在的任何基因都可能不存在于另一个单倍型上。这表明，通过重组加速进化的代价可能是单倍型上某些框架基因的丢失。
