Campbell Charles Frederick
Department of Surgery, Centre for Cancer Research and Cell Biology, Queen's University of Belfast, Belfast BT12 6BJ, Northern Ireland.
Methods Mol Biol. 2010;633:197-206. doi: 10.1007/978-1-59745-019-5_14.
Complex cell signal transduction mechanisms regulate intestinal epithelial shape, polarity, motility, organelles, cell membrane components as well as physical and mechanical properties to influence alimentary digestion, absorption, secretion, detoxification and fluid balance. Interactions between the epithelial cells and adjacent mesenchyme are central to intestinal homeostasis although the key regulatory molecules of specific differentiation steps remain unclear. Isolation and primary culture of heterotypic murine intestinal cells provides a model system for elucidation of essential molecular cross-talk between epithelium and mesenchyme that may provide several biological and practical advantages over transformed cell lines. An in vitro primary culture system for neonatal rat or mouse intestinal cells has been established that forms monolayers, expresses intestine-specific epithelial features including intestinal brush borders and appropriate hydrolase enzymes. Our studies confirm the promise of this method which may advance our understanding of heterotypic cellular interactions implicated in intestinal function and may provide important insights into the pathobiology of disease.
复杂的细胞信号转导机制调节肠道上皮的形状、极性、运动性、细胞器、细胞膜成分以及物理和机械特性，以影响营养物质的消化、吸收、分泌、解毒和液体平衡。上皮细胞与相邻间充质之间的相互作用对于肠道稳态至关重要，尽管特定分化步骤的关键调节分子仍不清楚。异型小鼠肠道细胞的分离和原代培养提供了一个模型系统，用于阐明上皮细胞与间充质之间必不可少的分子相互作用，这可能比转化细胞系具有若干生物学和实际优势。已经建立了一种用于新生大鼠或小鼠肠道细胞的体外原代培养系统，该系统形成单层，表达包括肠道刷状缘和适当水解酶在内的肠道特异性上皮特征。我们的研究证实了这种方法的前景，它可能会增进我们对与肠道功能相关的异型细胞相互作用的理解，并可能为疾病的病理生物学提供重要见解。
