Ye Hua, Wu Qiong, Zhu Yuzhen, Guo Cancan, Zheng Xuebao
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, 2 Wenming East Road, Zhanjiang, 524023, Guangdong, China,
Mol Biol Rep. 2014 Aug;41(8):5485-90. doi: 10.1007/s11033-014-3422-0. Epub 2014 Jun 4.
Ginsenoside Rh2 (GRh2) has been reported to have therapeutic effects on various diseases. However, whether it may also affect the recovery from ulcerative colitis remains unknown. Here we induced colitis in mice by dextran sulfate sodium (DSS) administration, and then treated the mice with GRh2. We found that GRh2-treated mice showed significant alleviation of the DSS-induced colitis. Moreover, significant increase in the activity of TGFβ signaling was detected in the GRh2-treated colon that had received DSS. To investigate whether there is a causative link among GRh2 treatment, TGFβ signaling augment and the cure of colitis, we gave the DSS-treated mice a combination of GRh2 and a specific TGFβ receptor I inhibitor, SB431542. SB431542 significantly decreased the activation of TGFβ signaling in the colon from the GRh2-administrated mice, and consequently attenuated the therapeutic effect of GRh2. Our data thus demonstrate that GRh2 may alleviate DSS-induced colitis via augmenting TGFβ signaling.
据报道，人参皂苷Rh2（GRh2）对多种疾病具有治疗作用。然而，它是否也会影响溃疡性结肠炎的恢复仍不清楚。在此，我们通过给予葡聚糖硫酸钠（DSS）诱导小鼠患结肠炎，然后用GRh2治疗这些小鼠。我们发现，用GRh2治疗的小鼠，其DSS诱导的结肠炎有显著缓解。此外，在接受DSS处理且用GRh2治疗的结肠中，检测到TGFβ信号传导活性显著增加。为了研究GRh2治疗、TGFβ信号增强与结肠炎治愈之间是否存在因果关系，我们给接受DSS处理的小鼠同时使用GRh2和一种特异性TGFβ受体I抑制剂SB431542。SB431542显著降低了给予GRh2的小鼠结肠中TGFβ信号的激活，从而减弱了GRh2的治疗效果。因此，我们的数据表明，GRh2可能通过增强TGFβ信号传导来缓解DSS诱导的结肠炎。
