Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi Province, 710032, China.
Invest New Drugs. 2011 Aug;29(4):637-45. doi: 10.1007/s10637-010-9402-x. Epub 2010 Mar 6.
Mucoepidermoid carcinoma (MEC) is the most common malignant tumor in salivary glands and high-grade MEC in particular demonstrates little response to chemotherapy which has been used largely for palliative treatment of metastatic disease. Baicalin, one of the main active compounds of Scutellaria baicalensis, possesses anti-inflammatory, antioxidant and antitumor properties. In the present study, we investigated the growth inhibiting and apoptosis-inducing effects of baicalin on a highly metastatic human mucoepidermoid carcinoma cell line Mc3 for the first time. Baicalin exerted dose- and time-dependent antiproliferative potential against Mc3 cells as assessed by MTT assay. Baicalin treatment of Mc3 cells resulted in an accumulation of cells at the G₀/G₁ and G₂/M phase with a concomitant decrease in cells processing to S phase as assessed by flow cytometry. Furthermore, baicalin induced apoptosis of Mc3 cells as determined by annexin V binding and PI dual staining, DNA fragmentation, nuclear condensation and in vivo tumor inhabitation. Rhodamine 123 assay indicated that baicalin caused cytotoxicity and induced apoptosis through decreasing the mitochondrial membrane potential in Mc3 cells. Our results suggest that baicalin seems to be very attractive as a new anticancer drug and a potential chemotherapeutic agent against human high-grade mucoepidermoid carcinoma.
黏液表皮样癌（MEC）是唾液腺最常见的恶性肿瘤，特别是高级别 MEC 对化疗反应不佳，化疗主要用于转移性疾病的姑息治疗。黄芩苷是黄芩的主要活性化合物之一，具有抗炎、抗氧化和抗肿瘤特性。在本研究中，我们首次研究了黄芩苷对高转移性人黏液表皮样癌 Mc3 细胞系的生长抑制和诱导凋亡作用。MTT 法评估结果显示，黄芩苷对 Mc3 细胞表现出剂量和时间依赖性的增殖抑制作用。流式细胞术检测结果表明，黄芩苷处理 Mc3 细胞后，G₀/G₁ 和 G₂/M 期细胞积累，而 S 期细胞减少。此外，通过 Annexin V 结合和 PI 双重染色、DNA 片段化、核浓缩和体内肿瘤抑制来确定黄芩苷诱导 Mc3 细胞凋亡。罗丹明 123 测定表明，黄芩苷通过降低 Mc3 细胞中的线粒体膜电位引起细胞毒性并诱导细胞凋亡。我们的研究结果表明，黄芩苷似乎是一种很有吸引力的新型抗癌药物，也是治疗人类高级别黏液表皮样癌的潜在化疗药物。
