EPR Center for Viable Systems, Department of Radiology, Dartmouth Medical School, 703 Vail, Hanover, NH 03755, USA.
Adv Exp Med Biol. 2010;662:149-56. doi: 10.1007/978-1-4419-1241-1_21.
Electron paramagnetic resonance (EPR) oximetry can be used to provide direct absolute measurements of pO(2) in living tissue using India ink as an O(2) reporter. In vivo measurements are made using low frequency (1.2 GHz) EPR spectroscopy and surface loop resonators, which enable measurements to be made at superficial sites through a non-invasive (after placing the ink in the tissues) and repeatable measurement procedure. Ongoing EPR oximetry studies in human subjects include measurement of subcutaneous pO(2) in the feet of healthy volunteers to develop procedures that could be used in the treatment of peripheral vascular disease and oximetry in tumors during courses of radiation and chemotherapy, to follow pO(2) so oxygen-dependent therapies can be optimized. In each case, we aim to provide quantitative measurements of tissue pO(2) which will aid physicians in the characterization of disease status and the effects of therapeutic measures, so that treatments can be applied with optimal effectiveness by taking into account the oxygen-dependent aspects of the therapy. The overall goal is to enhance clinical outcomes. Oximetry measurements of subcutaneous tissue on dorsal and plantar foot surfaces have been made in 9 volunteers, with measurements ongoing for each and the longest set of measurements carried out successfully over the last 5 years. Tumor oximetry measurements have been performed in tumor tissues of 10 patients during courses of radiation and chemotherapy. Tumor types include melanoma, basal cell, soft tissue sarcoma, and lymphoma, and measurement sites have ranged from the feet to the scalp. These studies demonstrate the feasibility of EPR oximetry in a clinical setting and the potential for more widespread use in the treatment of these and other oxygen-dependent diseases.
电子顺磁共振（EPR）血氧计可用于使用印度墨水作为 O2 指示剂直接提供活体组织中 pO2 的绝对测量值。体内测量使用低频（1.2GHz）EPR 光谱和表面环形谐振器进行，这使非侵入性（将墨水放置在组织中后）和可重复的测量程序能够在浅表部位进行测量。目前正在对人体进行 EPR 血氧计研究，包括测量健康志愿者脚部的皮下 pO2，以开发可用于治疗周围血管疾病的程序，以及在放射治疗和化学治疗过程中对肿瘤进行血氧计测量，以监测 pO2，以便优化氧依赖性治疗。在每种情况下，我们都旨在提供组织 pO2 的定量测量值，这将有助于医生对疾病状态和治疗措施的影响进行描述，以便在考虑治疗的氧依赖性方面的情况下，通过应用最佳效果的治疗方法来进行治疗。总体目标是提高临床效果。已经在 9 名志愿者的背部和足底足部表面的皮下组织上进行了血氧计测量，并且正在对每个人进行测量，并且在过去 5 年内成功地进行了最长的一组测量。在放射治疗和化学治疗过程中，对 10 名患者的肿瘤组织进行了肿瘤血氧计测量。肿瘤类型包括黑色素瘤、基底细胞癌、软组织肉瘤和淋巴瘤，测量部位从脚部到头皮不等。这些研究证明了 EPR 血氧计在临床环境中的可行性以及在治疗这些和其他氧依赖性疾病方面更广泛应用的潜力。
