Department of Biophysics and Cancer Biology, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University, 7 Gronostajowa St., 30-387, Krakow, Poland.
Department of Radiology, Geisel School of Medicine, Dartmouth College, 1 Rope Ferry Rd, Hanover, NH, 03755, USA.
Mol Imaging Biol. 2024 Jun;26(3):473-483. doi: 10.1007/s11307-023-01859-w. Epub 2023 Oct 2.
The goal of this work was to compare pO measured using both continuous wave (CW) and pulse electron paramagnetic resonance (EPR) spectroscopy. The Oxychip particle spin probe enabled longitudinal monitoring of pO in murine pancreatic tumor treated with gemcitabine during the course of therapy.
Pancreatic PanO2 tumors were growing in the syngeneic mice, in the leg. Five doses of saline in control animals or gemcitabine were administered every 3 days, and pO was measured after each dose at several time points. Oxygen partial pressure was determined from the linewidth of the CW EPR signal (Bruker E540L) or from the T measured using the electron spin echo sequence (Jiva-25™).
The oxygen sensitivity was determined from a calibration curve as 6.1 mG/mm Hg in CW EPR and 68.5 ms/mm Hg in pulse EPR. A slight increase in pO of up to 20 mm Hg was observed after the third dose of gemcitabine compared to the control. The maximum delta pO during the therapy correlated with better survival.
Both techniques offer fast and reliable oximetry in vivo, allowing to follow the effects of pharmaceutic intervention.
本研究旨在比较连续波（CW）和脉冲电子顺磁共振（EPR）光谱法测量的 pO 值。Oxychip 粒子自旋探针可在接受吉西他滨治疗的荷瘤小鼠的胰腺肿瘤中进行纵向监测 pO 值。
将同基因小鼠的胰腺 PanO2 肿瘤种植于腿部。对照组动物给予 5 次生理盐水，或每 3 天给予吉西他滨 1 次，每次给药后在多个时间点测量 pO 值。通过 CW EPR 信号的线宽（Bruker E540L）或使用电子自旋回波序列（Jiva-25™）测量的 T 值确定氧分压。
CW EPR 中的氧灵敏度校准曲线为 6.1 mG/mm Hg，脉冲 EPR 中的氧灵敏度校准曲线为 68.5 ms/mm Hg。与对照组相比，吉西他滨第三次给药后 pO 值略有升高，最高可达 20 mmHg。治疗过程中的最大 delta pO 值与更好的生存相关。
两种技术均可提供快速可靠的体内血氧测量，可用于监测药物干预的效果。
Zotero 插件
