Khan Nadeem, Williams Benjamin B, Hou Huagang, Li Hongbin, Swartz Harold M
EPR Center for Viable Systems, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Antioxid Redox Signal. 2007 Aug;9(8):1169-82. doi: 10.1089/ars.2007.1635.
Tissue oxygen plays a crucial role in maintaining tissue viability and in various diseases, including responses to therapy. Useful knowledge has been gained by methods that can give limited snapshots of tissue oxygen (e.g., oxygen electrodes) or evidence of a history of tissue hypoxia (e.g., EF5) or even indirect evidence by monitoring oxygen availability in the circulatory system (e.g., NMR methods). Each of these methods has advantages and significant limitations. EPR oximetry is a technique for direct measurement of tissue pO2, which has several advantages over the other existing methods for applications in which the parameter of interest is the pO2 of tissues, and information is needed over a time course of minutes to hours, and/or for repetitive measurements over days or weeks or years. The aim of this article is to provide an overview of EPR oximetry using particulates to readers who are not familiar with this technique and its potential in vivo and clinical applications. The data presented here are from the experiments currently being carried out in our laboratory. We are confident that in vivo EPR oximetry will play a crucial role in the understanding and clinical management of various pathologies in the years to come.
组织氧在维持组织活力以及包括对治疗反应在内的各种疾病中起着至关重要的作用。通过能够提供组织氧有限瞬间图像的方法（如氧电极）、组织缺氧历史证据的方法（如EF5），甚至通过监测循环系统中氧可用性的间接证据的方法（如核磁共振方法），已经获得了有用的知识。这些方法中的每一种都有优点和显著的局限性。电子顺磁共振血氧测定法是一种直接测量组织pO2的技术，对于那些感兴趣的参数是组织pO2、需要在数分钟至数小时的时间过程中获取信息，和/或需要在数天、数周或数年进行重复测量的应用而言，它相对于其他现有方法具有若干优势。本文的目的是向不熟悉该技术及其体内和临床应用潜力的读者提供使用微粒的电子顺磁共振血氧测定法概述。此处呈现的数据来自我们实验室目前正在进行的实验。我们相信，在未来几年中，体内电子顺磁共振血氧测定法将在理解和临床管理各种病症方面发挥关键作用。
