植物中DNA甲基化的原位分析。
In situ analysis of DNA methylation in plants.
作者信息
Kathiria Palak, Kovalchuk Igor
机构信息
Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
出版信息
Methods Mol Biol. 2010;631:41-8. doi: 10.1007/978-1-60761-646-7_5.
Epigenetic changes in the plant genome are associated with differential genome methylation, histone modifications, and the binding of various chromatin-binding factors. Methylation of cytosine residues is one of the most versatile mechanisms of epigenetic regulation. The analysis of DNA methylation can be performed in different ways. However, most of these procedures involve the extraction of chromatin from cells with further isolation and analysis of DNA. Modest success has been achieved in DNA methylation analysis in plant tissues in situ. Here, we present an in situ method for DNA methylation analysis, which has high sensitivity and good reproducibility.
植物基因组中的表观遗传变化与基因组甲基化差异、组蛋白修饰以及各种染色质结合因子的结合有关。胞嘧啶残基的甲基化是表观遗传调控最通用的机制之一。DNA甲基化分析可以通过不同方式进行。然而，这些方法大多涉及从细胞中提取染色质，并进一步分离和分析DNA。植物组织原位DNA甲基化分析已取得一定成功。在此，我们提出一种用于DNA甲基化分析的原位方法，该方法具有高灵敏度和良好的重现性。
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