使用胞嘧啶延伸测定法检测全基因组甲基化的变化。

Detection of changes in global genome methylation using the cytosine-extension assay.

作者信息

Boyko Alex, Kovalchuk Igor

机构信息

Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.

出版信息

Methods Mol Biol. 2010;631:33-9. doi: 10.1007/978-1-60761-646-7_4.

DOI:10.1007/978-1-60761-646-7_4

PMID:20204866

Abstract

Methylation is a reversible covalent chemical modification of DNA intended to regulate gene expression, genome stability, and chromatin structure. Although there are various methods of methylation analysis, most of them are either laborious or expensive, or both. Here, we describe a quick, inexpensive method for analysis of global genome methylation using a cytosine extension assay. The assay can be used for analysis of the total level of CpG, CNpG, and asymmetrical methylation in a given cell culture or in a plant tissue sample.

摘要

甲基化是一种旨在调控基因表达、基因组稳定性和染色质结构的DNA可逆共价化学修饰。尽管存在多种甲基化分析方法，但其中大多数要么耗时费力，要么成本高昂，或者两者兼具。在此，我们描述一种使用胞嘧啶延伸测定法快速、低成本分析全基因组甲基化的方法。该测定法可用于分析给定细胞培养物或植物组织样本中CpG、CNpG和不对称甲基化的总水平。