Danone Research - Centre for Specialised Nutrition, Wageningen, The Netherlands.
Int Immunopharmacol. 2010 May;10(5):619-25. doi: 10.1016/j.intimp.2010.02.014. Epub 2010 Mar 3.
The immune-modulatory effect of specific prebiotic oligosaccharides was shown in previous preclinical and clinical studies. To enhance the understanding of this effect, kinetic aspects of immune modulation and the correlation between microbiological and immunological parameters were investigated in a murine vaccination model. C57BL/6 mice were supplemented with short-chain galactooligosaccharides and long-chain fructooligosaccharides (ratio 9:1; Immunofortis()) in combination with pectin-derived acidic oligosaccharides. The timing of supplementation was varied around the primary (day 0) and secondary (day 21) vaccinations. Supplementation before the primary vaccination was necessary to increase delayed-type hypersensitivity responses (DTH) significantly at day 30. Supplementation after day 8 did not affect the DTH response at day 30, indicating that immune modulation occurred during the early phase. Therefore, correlation analysis of microbiological and immunological parameters was performed in a shortened experiment to focus on the early phase. At day 9 post-priming, the percentages of cecal lactobacilli were correlated to the DTH responses (p=0.01). Furthermore, the results suggested that yet unidentified factors may play a role. Additional analysis of intestinal Peyer's patch major lymphocyte populations did not show effects of supplementation. In conclusion, a specific oligosaccharide mixture was shown to exert its immune-modulatory effect during the early phase of a murine immune response. The results are consistent with a role of the microbiota and possibly other factors in oligosaccharide-induced immune modulation. Furthermore, the results demonstrate that it is critical to consider kinetic aspects of immune-modulatory and prebiotic effects in order to study their interaction in a meaningful way.
先前的临床前和临床研究表明，特定的益生元低聚糖具有免疫调节作用。为了更深入地了解这种作用，本研究在小鼠免疫接种模型中，从动力学角度研究了免疫调节以及微生物学和免疫学参数之间的相关性。将短链半乳糖低聚糖和长链果聚糖（比例 9:1；Immunofortis（））与果胶衍生的酸性低聚糖联合添加到 C57BL/6 小鼠中。补充的时间围绕着初次（第 0 天）和二次（第 21 天）接种进行变化。在初次接种前进行补充，可在第 30 天显著提高迟发型超敏反应（DTH）。在第 8 天后进行补充，则不会影响第 30 天的 DTH 反应，表明免疫调节发生在早期阶段。因此，在缩短的实验中，对微生物学和免疫学参数进行了相关性分析，重点关注早期阶段。在初次免疫后第 9 天，盲肠乳杆菌的百分比与 DTH 反应呈正相关（p=0.01）。此外，结果表明可能还有其他未知因素在起作用。对肠道派尔集合淋巴结主要淋巴细胞群的补充分析并未显示出影响。总之，特定的低聚糖混合物在小鼠免疫反应的早期阶段发挥其免疫调节作用。结果表明，在低聚糖诱导的免疫调节中，微生物群可能还有其他因素发挥作用。此外，结果表明，为了以有意义的方式研究它们的相互作用，必须考虑免疫调节和益生元作用的动力学方面。
