Shanker R, Dogra R K, Khanna S, Srivastava S N, Shukla L J, Gupta S, Katiyar J C
Immunotoxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Indian J Exp Biol. 1992 May;30(5):388-93.
Pathomorphological and immunological studies were carried out on rodents following oral administration of 0, 0.1, 0.25 and 0.5% (w/w) metanil yellow, mixed in diet, for 30 days. No significant change in hematologic parameters and histologic architecture of liver, kidney, mesenteric lymph node, thymus and urinary bladder was observed except for mild desquamation of intestinal villi and moderate changes in Peyer's patches of small intestine with higher doses. Among immunological parameters, significant enhancement in the primary humoral immune response (anti-SRBC IgM plaque forming cells of spleen) was observed with the lowest dose of metanil yellow while higher doses produced opposing effects. An elevated cutaneous delayed type hypersensitivity (DTH) reaction to SRBC was seen in 0.1% metanil yellow treated animals but higher doses did not influence the reaction. The treatment also caused changes in functional capabilities of macrophages. Although these immune alterations could hardly influence the local immunity of gut, as measured by the capacity of animals to cause rejection of Nippostrongylus brasiliensis parasite, the potential to modulate the immunity in general by metanil yellow however assumes considerable biological significance.
对啮齿动物进行了病理形态学和免疫学研究，这些动物在30天内口服了分别以0%、0.1%、0.25%和0.5%（w/w）的间硝基苯黄与饲料混合的食物。除了高剂量组出现轻度肠绒毛脱落和小肠派尔集合淋巴结中度变化外，未观察到血液学参数以及肝脏、肾脏、肠系膜淋巴结、胸腺和膀胱的组织学结构有显著变化。在免疫学参数方面，最低剂量的间硝基苯黄可使主要体液免疫反应（脾脏抗绵羊红细胞IgM斑块形成细胞）显著增强，而高剂量则产生相反作用。在接受0.1%间硝基苯黄处理的动物中，对绵羊红细胞的皮肤迟发型超敏反应（DTH）升高，但高剂量组未影响该反应。该处理还导致巨噬细胞功能能力发生变化。尽管这些免疫改变几乎不会影响肠道的局部免疫（通过动物排斥巴西日圆线虫寄生虫的能力来衡量），但间硝基苯黄总体上调节免疫的潜力具有相当大的生物学意义。
