Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
J Colloid Interface Sci. 2010 May 15;345(2):325-31. doi: 10.1016/j.jcis.2009.10.004. Epub 2009 Oct 13.
A polymer brush of 2-(2-methoxyethoxy)ethyl methacrylate (MDM) was prepared by atom transfer radical polymerization (ATRP) using a 11-(2-bromoisobutyroyloxy)undecyl moiety-carrying initiator covalently fixed to a glass substrate. An aqueous solution of the MDM polymer (E-PMDM), which had been prepared for comparison, turned to be opaque above certain temperature (26.2 °C for E-PMDM (M(n,GPC)=1.84×10(4))), which was corresponding to the lower critical solution temperature (LCST) of the polymer. The PMDM polymer brush accumulated on the glass surface also indicated temperature-responsive changes in contact angle of air bubble in the air-in-water system. Furthermore, non-specific adsorption of various proteins (bovine serum albumin (BSA), human immunoglobulin G (IgG) and bovine plasma fibrinogen (BPF)) to the surface of polymer brush on the glass plate was examined by the bicinchoninic acid method. The PMDM brush did not adsorb IgG and BPF significantly below the LCST of the polymer chain, whereas adsorbed a larger amount of the proteins above the LCST. A similar but less significant temperature-responsive adsorption was observed in the case of BSA. These results suggest usability of the temperature-responsive polymer-brushes with pendent ω-methoxy oligo(ethylene glycol) groups to coat various materials for bio-medical applications.
通过原子转移自由基聚合（ATRP），使用共价固定在玻璃基底上的带有 11-（2-溴异丁酰氧基）十一烷基部分的引发剂，制备了 2-（2-甲氧基乙氧基）乙基甲基丙烯酸酯（MDM）的聚合物刷。为了进行比较，制备了 MDM 聚合物的水溶液（E-PMDM），在高于一定温度（对于 E-PMDM（M(n,GPC)=1.84×10(4)）为 26.2°C）时，该溶液变得不透明，这对应于聚合物的低临界溶液温度（LCST）。在空气-水体系中，PMDM 聚合物刷在玻璃表面上的积累也表明了气泡接触角的温度响应变化。此外，通过双缩脲法检查了各种蛋白质（牛血清白蛋白（BSA）、人免疫球蛋白 G（IgG）和牛血浆纤维蛋白原（BPF））在玻璃盘上聚合物刷表面的非特异性吸附。在聚合物链的 LCST 以下，PMDM 刷不会显著吸附 IgG 和 BPF，而在 LCST 以上则吸附了更多的蛋白质。在 BSA 的情况下观察到了类似但不太明显的温度响应吸附。这些结果表明，带有末端 ω-甲氧基聚（乙二醇）基团的温度响应聚合物刷可用于涂覆各种用于生物医学应用的材料。
