经超高压匀浆预处理有利于甘蔗渣中纤维素酶的消化。
Pretreatment by ultra-high pressure explosion with homogenizer facilitates cellulase digestion of sugarcane bagasses.
机构信息
College of Life Science and Biotechnology, Guangxi University, 100 University Road, Nanning, Guangxi 530004, China.
出版信息
Bioresour Technol. 2010 Jul;101(14):5592-600. doi: 10.1016/j.biortech.2010.02.003. Epub 2010 Mar 4.
Sugarcane bagasse was treated with ultra-high pressure explosion (UHPE) in a homogenizer. UHPE significantly decreased the particle size and disrupt the microstructure of bagasse, which was changed from a rigid and highly ordered fibril to a distorted, poly-porous and "empty-inside" structure. UHPE decreased the crystallinity indexes (CrIs) from 54.8% (0 MPa) to 45.3% (100 MPa), and increased the accessible surface area (ASA) of bagasse nearly 3-fold. The effect of UHPE on composition was minor, only decreased the lignin slightly. However, UHPE increased enzymatic digestibility substantially from 29.5% (0 MPa) to 59.4% (100 MPa), and the microstructure disruption and thereby ASA increase were found to be the major factors to enhance the digestibility. UHPE combination with treatment of 0.5% NaOH at 125 degrees C for 120 min exhibited a significant delignification and increased enzymatic digestibility to 95.5% with 5.8 FPU cellulase/g solid, implying that UHPE combined with alkaline pretreatment can be a potential alternative for biomass pretreatment in biofuel production.
甘蔗渣在均相器中进行超高压爆炸（UHPE）处理。UHPE 显著降低了甘蔗渣的粒径并破坏了其微观结构，使其由刚性和高度有序的原纤维转变为变形、多孔和“中空”的结构。UHPE 将结晶度指数（CrIs）从 54.8%（0 MPa）降低至 45.3%（100 MPa），并使甘蔗渣的可及表面积（ASA）增加近 3 倍。UHPE 对成分的影响较小，仅略微降低了木质素含量。然而，UHPE 使酶解率从 29.5%（0 MPa）大幅提高至 59.4%（100 MPa），发现微观结构的破坏和 ASA 的增加是提高酶解率的主要因素。将 UHPE 与 0.5%NaOH 在 125°C 下处理 120 分钟的组合处理表现出显著的脱木质素作用，并使酶解率提高至 95.5%，用 5.8 FPU 纤维素酶/g 固体，这意味着 UHPE 与碱性预处理相结合可能是生物燃料生产中生物质预处理的一种潜在替代方法。
Zotero 插件