Department of Biology, Biomedical Sciences Stream, Addis Ababa University, PO Box 42524, Addis Ababa, Ethiopia.
Acta Trop. 2010 Jul-Aug;115(1-2):77-83. doi: 10.1016/j.actatropica.2010.02.003. Epub 2010 Mar 4.
In this study, 1034 faecal samples from patients with diarrhoea were screened for Cryptosporidium oocysts. Samples were collected from nine different regions in Ethiopia. Of these, 79 samples (7.6%) were positive for Cryptosporidium by modified Ziehl-Neelson staining. From all positive samples DNA was extracted and PCR amplification of the COWP, SSU-rRNA and GP60 gene fragments was performed. A total of 41 samples (52%) were positive in any of the three typing methods. The majority of isolates (39 of 41) was identified as Cryptosporidium parvum, with one Cryptosporidium hominis and one mixed infection. Sequencing of the GP60 gene fragments of 13 isolates resulted in three different subgenotypes of C. parvum, all belonging to the zoonotic subtype family IIa and one subtype of C. hominis (Ib). These data identify C. parvum as the major cause of human cryptosporidiosis in Ethiopia and suggest a zoonotic transmission of the disease in contrast to reports from other developing countries.
在这项研究中，从腹泻患者的 1034 份粪便样本中筛选隐孢子虫卵囊。样本来自埃塞俄比亚的九个不同地区。其中，79 份样本（7.6%）经改良齐氏染色法呈隐孢子虫阳性。从所有阳性样本中提取 DNA，并对 COWP、SSU-rRNA 和 GP60 基因片段进行 PCR 扩增。三种分型方法中共有 41 份（52%）呈阳性。大多数分离株（41 个中的 39 个）鉴定为微小隐孢子虫，1 个为隐孢子虫人源种，1 个为混合感染。对 13 个分离株的 GP60 基因片段进行测序，结果产生了 3 种不同的微小隐孢子虫亚基因型，均属于动物源亚型家族 IIa，1 种为人源种（Ib）。这些数据表明微小隐孢子虫是埃塞俄比亚人类隐孢子虫病的主要病因，并提示该病在埃塞俄比亚存在动物源性传播，与其他发展中国家的报告不同。
