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[使用噬斑法从肉类、器官和肉制品中分离奥耶斯基氏病病毒]

[Use of the plaque method for isolating Aujeszky's disease virus from meat, organs and meat products].

作者信息

Kunev Zh

出版信息

Vet Med Nauki. 1977;14(6):79-83.

PMID:202066
Abstract

Comparative virologic studies were carried out to isolate the virus of Aujesky's disease from meat, viscera, and raw smoked products by the plaque method as modified by Sullivan and Read as well as by the method with which the presence of the virus is recorded visually by the cytopathic effect observed in the infected cell monolayer. It was found that at the veterinary and sanitary inspection of meat and meat products produced from diseases or survived animals the modified plaque method proved more suitable as against the other method. The former made it possible to determine directly and at low titers the virus of Aujeszky's disease for the needs of research and practical activities associated with rendering the meat harmless as coming from diseased pigs.

摘要

开展了比较病毒学研究,采用经沙利文和里德改良的蚀斑法,以及通过观察感染细胞单层中的细胞病变效应直观记录病毒存在的方法,从肉类、内脏和生熏制产品中分离奥耶斯基病病毒。结果发现,在对患病或康复动物生产的肉类和肉制品进行兽医卫生检查时,改良蚀斑法比另一种方法更适用。对于与使患病猪的肉无害化相关的研究和实际活动而言,前者能够直接且以低滴度测定奥耶斯基病病毒。

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