National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, 480, Anyang City, Gyeonggi-do 430-824, Republic of Korea.
Res Vet Sci. 2010 Aug;89(1):1-4. doi: 10.1016/j.rvsc.2010.01.011. Epub 2010 Mar 5.
Fifty-four quinupristin/dalfopristin-resistant Enterococcus faecium (QDREF) isolated from chickens and pigs during 2002-2003 in Korea were screened by PCR for the presence of streptogramin resistance genes vatD, vatE, and vgbA, and macrolide resistance gene ermB. None of the QDREF isolates carried vgbA and vatD genes, while vatE and ermB were detected in 9.2% and 74% of the isolates, respectively. Twenty-six percent (14/54) of the QDREF isolates contained none of the resistance determinants tested. Pulsed-field gel electrophoresis (PFGE) patterns revealed high heterogeneity: 47 different patterns for 54 QDREF evaluated. Identical PFGE types were observed in two pairs of chicken isolates and a pair of pig isolates, respectively, but chicken isolates did not share PFGE pattern with pig isolates, suggesting clonal spread of QDREF strain between the same species of animals but not between different species of animals. This is the first report, to our knowledge, of vatE-positive E. faecium isolates and also the first evidence of clonal spread of QDREF strain between animals in Korea.
2002-2003 年期间，从韩国的鸡和猪中分离出 54 株耐奎奴普丁/达福普汀肠球菌（QDREF），通过 PCR 筛选这些菌株以检测存在链阳性菌素抗性基因 vatD、vatE 和 vgbA 以及大环内酯类抗性基因 ermB。QDREF 分离株均不携带 vgbA 和 vatD 基因，而 vatE 和 ermB 分别在 9.2%和 74%的分离株中检测到。26%（14/54）的 QDREF 分离株不含有检测到的任何抗性决定因素。脉冲场凝胶电泳（PFGE）图谱显示出高度的异质性：对 54 株 QDREF 进行评估，共发现 47 种不同的 PFGE 类型。在两对鸡分离株和一对猪分离株中观察到相同的 PFGE 类型，但鸡分离株与猪分离株的 PFGE 模式不相同，这表明 QDREF 菌株在同种动物之间发生了克隆传播，但在不同种动物之间没有发生。据我们所知，这是首次报道 vatE 阳性肠球菌分离株，也是首次在韩国证明 QDREF 菌株在动物之间发生克隆传播的证据。
