Yan Xiao-Mei, Wang Jing, Tao Xiao-Xia, Jia Hong-Bing, Meng Fan-Liang, Yang Hui, You Yuan-Hai, Zheng Bo, Hu Yuan, Bu Xiao-Xia, Zhang Jian-Zhong
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Department of Clinical Diagnosis, China-Japan Friendship Hospital, Beijing, China.
Front Microbiol. 2021 Jun 4;12:667415. doi: 10.3389/fmicb.2021.667415. eCollection 2021.
(E) is a pleuromutilin, lincosamide, and streptogramin A (PLSA phenotype) resistance gene that was first described in and was thought to have been transferred from sp. This study aimed to elucidate the prevalence of the (E) gene among isolates at a tertiary teaching hospital and to evaluate the transferability of the (E) gene from to . A total of 96 strains isolated from one hospital in Beijing in 2013 were analysed for quinupristin-dalfopristin (QDA) resistance genes, and multilocus sequence typing (MLST) was performed. The transferability of QDA resistance between ten strains and four strains was determined by filter mating. Genome sequencing of the transconjugant was performed. A total of 46 isolates (46/96, 47.92%) tested positive for (E), while two isolates (2/96, 2.08%) tested positive for (A). Thirty-six (E)-positive strains (36/46, 78.3%) belonged to ST78. Among 40 mating tests, (E) was successfully transferred through one conjugation at a frequency of 1.125 × 10 transconjugants per donor. The QDA resistance of the transconjugant N7435-R3645 was expressed at a higher level (MIC = 16 mg/L) than that of the parent strain (MIC = 0.38 mg/L). Next-generation sequencing (NGS) analysis of the transconjugant N7435-R3645 showed that the complete sequence of the (E)-carrying plasmid pN7435-R3645 had a size of 92,396 bp and a G + C content of 33% (accession no. MT022086). The genetic map of pN7435-R3645 had high nucleotide similarity and shared the main open reading frame (ORF) features with two plasmids: pMG1 (AB206333.1) and LS170308 (CP025078.1). The gene of pN7435-R3645 showed 100% identity with that of pMG1, although it did not belong to the 1-19 family but instead a unique family. Multiple antibiotic resistance genes, including (E), and (B), (B), -Ia, and (B), were present on the plasmid. In conclusion, an (E)-carrying plasmid that can be transferred by conjugation from to was identified. This multidrug resistance (MDR) pMG1-like plasmid may act as a vector in the dissemination of antimicrobial resistance among species.
（E）是一种截短侧耳素、林可酰胺和链阳菌素A（PLSA表型）抗性基因，于[具体时间]首次被描述，被认为是从[具体菌种]转移而来。本研究旨在阐明某三级教学医院[具体菌种]分离株中（E）基因的流行情况，并评估（E）基因从[供体菌种]向[受体菌种]的可转移性。对2013年从北京一家医院分离的96株[具体菌种]菌株进行了奎奴普丁-达福普汀（QDA）抗性基因分析，并进行了多位点序列分型（MLST）。通过滤膜杂交确定了10株[供体菌种]菌株和4株[受体菌种]菌株之间QDA抗性的可转移性。对转接合子进行了全基因组测序。共有46株[具体菌种]分离株（46/96，47.92%）（E）基因检测呈阳性，而2株分离株（2/96，2.08%）（A）基因检测呈阳性。36株（E）基因阳性菌株（36/46，78.3%）属于ST78。在40次杂交试验中，（E）基因通过一次接合成功转移，转移频率为每供体1.125×10个转接合子。转接合子N7435-R3645的QDA抗性表达水平（MIC = 16 mg/L）高于亲本[具体菌种]菌株（MIC = 0.38 mg/L）。对转接合子N7435-R3645的二代测序（NGS）分析表明，携带（E）基因的质粒pN7435-R3645的完整序列大小为92396 bp，G + C含量为33%（登录号MT022086）。pN7435-R3645的遗传图谱与两个质粒：[具体菌种]pMG1（AB206333.1）和[具体菌种]LS170308（CP025078.1）具有高度核苷酸相似性，并共享主要开放阅读框（ORF）特征。pN7435-R3645的[具体基因]与pMG1的[具体基因]具有100%的同一性，尽管它不属于1-19家族，而是属于一个独特的[具体家族]。质粒上存在多个抗生素抗性基因，包括（E）、[其他基因]和（B）、（B）、-Ia以及（B）。总之，鉴定出了一种可通过接合从[供体菌种]转移至[受体菌种]的携带（E）基因的质粒。这种多药耐药（MDR）pMG1样质粒可能在[具体菌种]间抗菌药物抗性传播中充当载体。
