Department of Perinatal Medicine Pregnancy Research Centre, Royal Women's Hospital, Parkville, 3052 Victoria, Australia.
Placenta. 2010 Apr;31(4):347-50. doi: 10.1016/j.placenta.2010.02.008. Epub 2010 Mar 6.
Measuring trophoblast function involves performing end-point assays that represent the response at a single time point. New technology from Roche Applied Science enables continuous monitoring of cells in real-time using specialized culture dishes containing micro-electrodes. The xCELLigence System allows continuous measurement and quantification of cell adhesion, proliferation, migration and invasion, thus creating a true picture of trophoblast function. Lag and log growth phases can be determined thus pinpointing optimal times to treat and harvest cells. Use of this system will provide valuable insights into trophoblast functions as well as the behaviour of other cell types found at the maternal-fetal interface.
测量滋养层功能涉及进行终点测定，这些测定代表了在单一时间点的反应。罗氏应用科学的新技术可以使用含有微电极的特殊培养皿实时连续监测细胞。xCELLigence 系统可以连续测量和定量细胞黏附、增殖、迁移和侵袭，从而真实反映滋养层功能。可以确定迟滞和对数生长阶段，从而确定最佳的治疗和收获细胞的时间。该系统的使用将为了解滋养层功能以及母胎界面上其他细胞类型的行为提供有价值的见解。
