Department of Obstetrics & Gynaecology, Royal Women's Hospital, University of Melbourne, Parkville, VIC, Australia.
Department of Pharmacology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Methods Mol Biol. 2024;2728:149-161. doi: 10.1007/978-1-0716-3495-0_13.
Analyses of trophoblast behavior in vitro are widely used to investigate and understand the trophoblast dysfunction associated with pregnancy pathologies. Key behaviors critical for trophoblast function include adhesion, proliferation, migration, and invasion. Traditionally, these behaviors have been measured using time-consuming single timepoint or endpoint assays. xCELLigence is a breakthrough technology that utilizes gold electrodes embedded in cell culture plates to collect electrical impedance measurements and monitor cell attachment, spreading, movement, and growth. It enables trophoblast functions to be quantitatively monitored in real-time, providing new insights into trophoblast behavior.
体外滋养层行为分析广泛用于研究和了解与妊娠病理相关的滋养层功能障碍。对滋养层功能至关重要的关键行为包括黏附、增殖、迁移和侵袭。传统上，这些行为是使用耗时的单次时间点或终点测定法来测量的。xCELLigence 是一项突破性技术，它利用嵌入细胞培养板的金电极收集电阻抗测量值，并监测细胞附着、铺展、运动和生长。它能够实时定量监测滋养层功能，为了解滋养层行为提供新的视角。
