Chemistry Department, Analytical Chemistry Division, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
J Pharm Biomed Anal. 2010 Sep 5;52(5):747-51. doi: 10.1016/j.jpba.2010.02.009. Epub 2010 Feb 13.
A new and simple method for the direct and simultaneous determination of Sb(III) and Sb(V) in meglumine antimoniate, the first-choice drug for leishmaniasis treatment, was developed. Speciation analysis was carried out using the quantitative separation of inorganic trivalent and pentavalent antimony on Dowex 1 x 4 resin from 1.5 mol l(-1) hydrochloric acid solution. The inductively coupled plasma optical emission spectrometry (ICP-OES) was used for determination of antimony. The interfering effects of As, Bi, Cd, Cu, Mn, Pb and Zn were examined and only Bi was found to be a significant interferent. The liberation of Sb(V) and Sb(III) from organoantimonial compounds without changing of oxidation state was carried out by means of 1.5 mol l(-1) hydrochloric acid solution. The spike recovery values obtained for Sb(III) in pharmaceutical sample varied from 92 to 100%. The method was successfully applied for the direct determination of antimony(III) and of antimony(V) in meglumine antimoniate.
开发了一种新的、简单的方法，可直接同时测定葡庚糖酸锑钠（治疗利什曼病的首选药物）中的三价锑和五价锑。采用 Dowex 1 x 4 树脂从 1.5 mol/L 盐酸溶液中定量分离无机三价和五价锑，进行形态分析。采用电感耦合等离子体光学发射光谱法（ICP-OES）测定锑。考察了砷、铋、镉、铜、锰、铅和锌的干扰影响，发现只有铋是一个显著的干扰。通过 1.5 mol/L 盐酸溶液将有机锑化合物中的五价锑和三价锑释放出来，而不改变氧化态。在药物样品中，三价锑的加标回收率为 92%至 100%。该方法成功地应用于直接测定葡庚糖酸锑钠中的三价锑和五价锑。
