Bundesanstalt für Arbeitschutz und Arbeitsmedizin, D-10317 Berlin, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):237-241. doi: 10.1099/ijs.0.021675-0. Epub 2010 Mar 5.
Two Gram-stain-positive, non-motile, non-spore-forming cocci (strains MK-7(T) and MPA-33(T)) were isolated from poultry houses. Strain MK-7(T) was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. Strain MPA-33(T) was isolated from the air of a turkey house on TSA after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, both strains were shown to belong to the genus Jeotgalicoccus; MK-7(T) was most closely related to Jeotgalicoccus psychrophilus YKJ-115(T) (99.3 % similarity) and MPA-33(T) was most closely related to Jeotgalicoccus halotolerans YKJ-101(T) (98.8 %). The quinone system of MK-7(T) was composed of equal amounts of menaquinones MK-7 and MK-6 and that of MPA-33(T) contained 76 % MK-7 and 24 % MK-6. The polar lipid profile of strain MK-7(T) consisted of the major compounds diphosphatidylglycerol and phosphatidylglycerol and six unidentified lipids present in minor to moderate amounts. In strain MPA-33(T), diphosphatidylglycerol was the single predominant lipid, whereas phosphatidylglycerol was detected in moderate amounts. In addition, one unidentified phospholipid and four unidentified lipids were detected. Fatty acid profiles with iso-15 : 0 and anteiso-15 : 0 as major fatty acids supported the affiliation of the strains to the genus Jeotgalicoccus. The results of physiological and biochemical tests as well as DNA-DNA hybridizations allowed clear phenotypic differentiation of strains MK-7(T) and MPA-33(T) from the most closely related species. Strains MK-7(T) and MPA-33(T) therefore represent novel species, for which the names Jeotgalicoccus coquinae sp. nov. (type strain MK-7(T) =DSM 22419(T) =CCM 7682(T) =CCUG 57956(T)) and Jeotgalicoccus aerolatus sp. nov. (type strain MPA-33(T) =DSM 22420(T) =CCM 7679(T) =CCUG 57953(T)) are proposed.
从家禽养殖场中分离到两株革兰氏阳性、非运动、非孢子形成的球菌（菌株 MK-7(T) 和 MPA-33(T)）。菌株 MK-7(T) 是从一种用于养鸭场的母鸭食品补充剂——贻贝类中分离出来的，它是从海洋肉汤琼脂上分离出来的。菌株 MPA-33(T) 是从火鸡舍的空气中通过过滤采样后在 TSA 上分离出来的。根据 16S rRNA 基因序列相似性研究，这两株菌均属于杰奥特加西亚菌属；MK-7(T) 与 Jeotgalicoccus psychrophilus YKJ-115(T)（相似度 99.3%）最为相似，而 MPA-33(T) 与 Jeotgalicoccus halotolerans YKJ-101(T)（相似度 98.8%）最为相似。MK-7(T) 的醌类系统由等量的menaquinone MK-7 和 MK-6 组成，而 MPA-33(T) 的醌类系统则含有 76%的 MK-7 和 24%的 MK-6。菌株 MK-7(T) 的极性脂谱由主要化合物双磷脂酰甘油和磷脂酰甘油以及六种含量较少的未知脂类组成。在菌株 MPA-33(T) 中，双磷脂酰甘油是唯一的主要脂质，而磷脂酰甘油的含量适中。此外，还检测到一种未知的磷脂和四种未知的脂质。以 iso-15:0 和 anteiso-15:0 为主要脂肪酸的脂肪酸图谱支持菌株属于杰奥特加西亚菌属。生理生化试验和 DNA-DNA 杂交试验的结果允许对菌株 MK-7(T) 和 MPA-33(T) 与最密切相关的种进行清晰的表型区分。因此，菌株 MK-7(T) 和 MPA-33(T) 代表了新的种，分别命名为 Jeotgalicoccus coquinae sp. nov.（模式菌株 MK-7(T) =DSM 22419(T) =CCM 7682(T) =CCUG 57956(T)）和 Jeotgalicoccus aerolatus sp. nov.（模式菌株 MPA-33(T) =DSM 22420(T) =CCM 7679(T) =CCUG 57953(T)）。
