Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2010 Jun;60(Pt 6):1307-1310. doi: 10.1099/ijs.0.013623-0. Epub 2009 Aug 10.
A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2(T), was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2(T) was able to grow in the presence of 0-23 % (w/v) NaCl and at pH 4.5-10.0 and 5-42 degrees C; optimum growth was observed with 3-8 % (w/v) total salts and at pH 6.5-8.0 and 28-37 degrees C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2(T) to the genus Jeotgalicoccus. The predominant menaquinone of strain NY-2(T) was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C(15 : 0) (49.0 %) and anteiso-C(15 : 0) (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2(T) was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2(T) formed a coherent cluster with Jeotgalicoccus marinus JSM 076033(T), Jeotgalicoccus halotolerans YKJ-101(T) and Jeotgalicoccus psychrophilus YKJ-115(T). Phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2(T) (=CCTCC AB 208288(T) =JCM 15687(T)) should be classified as the type strain of a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus huakuii sp. nov. is proposed.
一株革兰氏阳性、氧化酶和过氧化氢酶阳性、不运动、不产孢、耐盐、球形的细菌，被命名为 NY-2(T) 菌株，从中国山东省的海滨土壤样本中分离得到。NY-2(T) 菌株能够在 0-23%（w/v）NaCl 存在下生长，在 pH4.5-10.0 和 5-42°C 下生长；在 3-8%（w/v）总盐和 pH6.5-8.0 及 28-37°C 下生长最佳。包括脂肪酸图谱、甲萘醌和极性脂类在内的化学分类学分析支持 NY-2(T) 菌株属于杰奥特盖拉氏菌属。NY-2(T) 菌株的主要甲萘醌是甲萘醌 7（MK-7）（100%），主要细胞脂肪酸是异-C15：0（49.0%）和anteiso-C15：0（19.6%）。细胞极性脂类为磷脂酰甘油、双磷脂酰甘油和几种未鉴定的磷脂。NY-2(T) 菌株的 DNA G+C 含量为 36.8mol%。基于 16S rRNA 基因序列分析，NY-2(T) 菌株与 Marinehalobacillus marinus JSM 076033(T)、Jeotgalicoccus halotolerans YKJ-101(T) 和 Jeotgalicoccus psychrophilus YKJ-115(T) 形成了一个连贯的聚类。系统发育分析、DNA-DNA 同源性数据、表型特征和化学分类数据表明，NY-2(T) 菌株（= CCTCC AB 208288(T) = JCM 15687(T)）应归类为杰奥特盖拉氏菌属的一个新种的模式株，因此建议将其命名为杰奥特盖拉氏菌 huakuii 种。
