Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):231-236. doi: 10.1099/ijs.0.021790-0. Epub 2010 Mar 5.
Two red-pigmented, strictly aerobic, pleomorphic rod-shaped and extremely halophilic archaea, designated strains HST01-2R(T) and HST03(T), were isolated from salt in a fish sauce sample from Thailand. The novel strains grew optimally at 37 °C, pH 7.0, and in the presence of 20-25 % (w/v) NaCl. The DNA G+C contents of the isolates were 61.6-62.2 mol%. Phylogenetic analysis based on a comparison of 16S rRNA gene sequences revealed that strains HST01-2R(T) and HST03(T) were placed in the radiation of species of the genus Haloarcula. The chemotaxonomic properties of the two strains, i.e. the presence of MK-8 and MK-8(H(2)) as the major menaquinone components and C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a triglycosyl diether as major polar lipids, supported the assignment of the two strains to the genus Haloarcula. Nevertheless, several phenotypic features and the low DNA-DNA relatedness between the two strains and related species of the genus Haloarcula (13.4-46.9 %) enabled the strains to be distinguished from each other and from recognized species. Therefore, strains HST01-2R(T) and HST03(T) represent two novel species in the genus Haloarcula, for which the names Haloarcula salaria sp. nov. and Haloarcula tradensis sp. nov. are proposed, respectively. The type strains are HST01-2R(T) (=BCC 40029(T)=JCM 15759(T)=PCU 313(T)) and HST03(T) (=BCC 40030(T)=JCM 15760(T)=PCU 314(T)).
两株红色、严格需氧、多形杆状且极度嗜盐的古菌，分别命名为 HST01-2R(T) 和 HST03(T)，从泰国鱼露样本中的盐中分离得到。新菌株在 37°C、pH7.0 和 20-25%（w/v）NaCl 存在下生长最佳。分离株的 DNA G+C 含量为 61.6-62.2 mol%。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 HST01-2R(T) 和 HST03(T) 被放置在 Haloarcula 属的物种辐射中。两株菌的化学分类特征，即主要类异戊二烯醌成分 MK-8 和 MK-8(H(2))以及磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和三糖脂醚的 C20C20 衍生物作为主要极性脂，支持将这两株菌分配到 Haloarcula 属中。然而，一些表型特征以及两株菌与 Haloarcula 属的相关种（13.4-46.9%）之间的低 DNA-DNA 相关性使得它们能够彼此区分，并与公认的种区分开来。因此，菌株 HST01-2R(T) 和 HST03(T) 代表 Haloarcula 属中的两个新种，分别命名为 Haloarcula salaria sp. nov. 和 Haloarcula tradensis sp. nov.。模式菌株为 HST01-2R(T)（=BCC 40029(T)=JCM 15759(T)=PCU 313(T)）和 HST03(T)（=BCC 40030(T)=JCM 15760(T)=PCU 314(T)）。
