1Laboratory Molecules of Communication and Adaptation of Microorganisms (MCAM) UMR 7245 CNRS-MNHN), Muséum national d'Histoire naturelle, Centre national de la Recherche scientifique (CNRS), CP 54, 57 rue Cuvier, 75005 Paris, France.
2Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA-CNRS-Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
Int J Syst Evol Microbiol. 2019 Mar;69(3):732-738. doi: 10.1099/ijsem.0.003211. Epub 2019 Jan 10.
A halophilic organism, SWO25, was isolated from water sampled in Algeria at the salt lake (sebkha) of Ouargla. The novel strain stained Gram-negative, and cells were pleomorphic with a red pigmentation. Strain SWO25 grew optimally at 35-45 °C, at pH 6.0-8.0 and 0.05-0.25 M MgCl2 concentrations. Cells were extremely halophilic, with optimal growth at 4.3-5.1 M NaCl. The predominant membrane polar lipids were C20C20 glycerol diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and diglycosyl diether. The major respiratory menaquinone component was MK-8. Cells were highly tolerant to the presence of decane and isooctane in the growth medium. Chemotaxonomic properties supported the assignment of strain SWO25 to the genus Haloarcula. The DNA G+C content was 61.1mol%. DNA-DNA hybridization and phylogenetic analyses of the 16S rRNA and rpoB' genes showed that strain SWO25 is distinct from known Haloarcula species. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, we describe a novel species of the genus Haloarcula, for which the name Haloarculasebkhae sp. nov. is proposed. The type strain is SWO25 (=CIP 110583=JCM 19018).
一种嗜盐微生物，SWO25，从阿尔及利亚沃特湖（盐沼）采集的水样中分离出来。该新菌株革兰氏染色阴性，细胞呈多形性，有红色色素。SWO25 菌株在 35-45°C、pH6.0-8.0 和 0.05-0.25 M MgCl2 浓度下生长最佳。细胞极度嗜盐，最佳生长盐度为 4.3-5.1 M NaCl。主要的膜极性脂是 C20C20 甘油二醚衍生的磷脂酰甘油、磷脂酰甘油磷酸、磷脂酰甘油硫酸酯、三糖基二醚和二糖基二醚。主要呼吸menaquinone 成分是 MK-8。细胞对生长培养基中癸烷和异辛烷的存在具有高度耐受性。化学分类学特性支持将 SWO25 菌株归属于 Haloarcula 属。DNA G+C 含量为 61.1mol%。16S rRNA 和 rpoB'基因的 DNA-DNA 杂交和系统发育分析表明，SWO25 菌株与已知的 Haloarcula 种不同。基于表型、化学分类学、基因型和系统发育数据，我们描述了一种新的 Haloarcula 属物种，建议将其命名为 Haloarculasebkhae sp. nov.。模式菌株为 SWO25(=CIP 110583=JCM 19018)。
