Peking University Third Hospital, Peking University Eye Center, Beijing, China.
Invest Ophthalmol Vis Sci. 2010 Jul;51(7):3742-8. doi: 10.1167/iovs.08-3314. Epub 2010 Mar 5.
PURPOSE. To investigate the effects and possible mechanisms of rat bone marrow mesenchymal stem cell (BMSC) transplantation on the light-damaged retinal structure and the apoptosis of photoreceptors. METHODS. DAPI-labeled BMSCs were transplanted into the subretinal space of light-damaged Sprague-Dawley rats 10 days after exposure. BMSCs were cultivated with the supernatant of homogenized retina (SHR). RESULTS. The outer nuclear layer (ONL) contained significantly more cells and the percentage of apoptotic ONL cells was significantly reduced in the BMSC transplantation group than in the phosphate-buffered solution injection group or the light damage group. Most DAPI-labeled BMSCs expressed brain-derived neurotrophic factor (BDNF). There was elevated basic fibroblast growth factor (bFGF) and BDNF immunoreactivity in the retinas of the BMSC transplantation group compared with the light damage group. In vitro culture showed that 10% of BMSCs changed from fusiform shape to multipolar shape. A fraction of cells expressed MAP2 or glial fibrillary acidic protein, and some cells expressed bFGF or BDNF when cultivated with light-damaged SHR for 7 days. CONCLUSIONS. BMSC subretinal transplantation could inhibit photoreceptor apoptosis and slow down retinal damage in light-damaged eyes. BMSCs could express bFGF (in vitro) and BDNF (in vitro and in vivo), pointing to potential trophic and protective effects on light-damaged retinas.
目的。研究大鼠骨髓间充质干细胞（BMSC）移植对光损伤视网膜结构和光感受器细胞凋亡的影响及其可能的机制。方法。光损伤后 10 天，将 DAPI 标记的 BMSC 移植到光损伤 Sprague-Dawley 大鼠的视网膜下腔。BMSC 与匀浆视网膜上清液（SHR）一起培养。结果。与磷酸盐缓冲液注射组或光损伤组相比，BMSC 移植组的外核层（ONL）中含有更多的细胞，且 ONL 细胞凋亡的百分比显著降低。大多数 DAPI 标记的 BMSC 表达脑源性神经营养因子（BDNF）。BMSC 移植组的视网膜中碱性成纤维细胞生长因子（bFGF）和 BDNF 免疫反应性明显升高。与光损伤组相比，体外培养显示 10%的 BMSC 从梭形变为多极形。部分细胞表达 MAP2 或神经胶质纤维酸性蛋白，当与光损伤 SHR 共培养 7 天时，部分细胞表达 bFGF 或 BDNF。结论。BMSC 视网膜下移植可抑制光感受器细胞凋亡，减缓光损伤眼的视网膜损伤。BMSC 可以表达 bFGF（体外）和 BDNF（体外和体内），这表明对光损伤视网膜具有潜在的营养和保护作用。
