Ekelund S, Påby P
Klinisk-Kemisk afdeling, Aalborg Sygehus, Denmark.
Scand J Clin Lab Invest. 1991 Feb;51(1):67-71.
A high-performance liquid chromatography (HPLC) method for creatinine has been developed. The method is based on ion-pair chromatography. The method is precise and easy to perform. Recoveries from enriched sera were from 98-105% with a mean of 100.5%. At 28 mumol l-1, 52 mumol l-1 and 487 mumol l-1 within-series standard deviations of 2 mumol l-1, 2 mumol l-1 and 10 mumol l-1, respectively were obtained. At 102 mumol l-1 among-series standard deviation of 6 mumol l-1 was obtained. Comparisons between HPLC and autoanalyser (Jaffé reaction) methods showed that the autoanalyser plasma and serum results, which are known to be sensitive to 'pseudocreatinines', varied between 93-217% of the HPLC results. Comparisons between HPLC, autoanalyser and enzymatic methods showed that the methods agree well for urine samples. The method presented appears suitable as a secondary reference method.
已开发出一种用于测定肌酐的高效液相色谱（HPLC）方法。该方法基于离子对色谱法。该方法精确且易于操作。富集血清的回收率为98 - 105%，平均回收率为100.5%。在28 μmol l-1、52 μmol l-1和487 μmol l-1浓度下，系列内标准差分别为2 μmol l-1、2 μmol l-1和10 μmol l-1。在102 μmol l-1浓度下，系列间标准差为6 μmol l-1。HPLC法与自动分析仪（Jaffé反应）法的比较表明，已知对“假肌酐”敏感的自动分析仪血浆和血清结果在HPLC结果的93 - 217%之间变化。HPLC法、自动分析仪法和酶法之间的比较表明，这些方法对尿液样本的结果吻合良好。所提出的方法似乎适合作为二级参考方法。
