采用离子对反相液相色谱法测定人血清中的精氨琥珀酸裂解酶。
Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography.
作者信息
Canepari S, Carunchio V, Girelli A M, Messina A
机构信息
Department of Chemistry, University of Rome La Sapienza, Italy.
出版信息
Biomed Chromatogr. 1995 Jul-Aug;9(4):171-4. doi: 10.1002/bmc.1130090404.
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.
报道了一种基于离子对反相机制分离酶促反应混合物成分的精氨酸琥珀酸裂解酶新检测方法。通过对生成的富马酸盐进行紫外检测，直接进样后测定酶活性。色谱分析时间为8分钟，检测限达到0.4 U/L。该高效液相色谱法具有高度的准确性、灵敏性和精密度。该简单程序使该方法适用于人血清样品中精氨酸琥珀酸裂解酶活性的常规测定。
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