The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
J Microbiol Biotechnol. 2010 Feb;20(2):383-90.
A newly isolated active producer of raw starch digesting amyloltic enzymes, Rhizopus microsporus var. chinensis CICIM-CU F0088 was screened and identified by morphological characteristics and molecular phylogenetic analysis. This fungus was isolated from the soil of Chinese glue pudding mill, and produced high levels of amylolytic activity under solid state fermentation with supplementation of starch and wheat bran. Results of thin layer chromatography showed there are two kinds of amyloltic enzymes formed by this strain, including one alpha-amylase and two glucoamylases. It was found that the two glucoamylases can digest raw corn starch in the electron microscope experiments, and has an optimal temperature of 70 degrees Celsius. These results signified that amyloltic enzymes secreted by strain of Rhizopus microsporus var. chinensis CICIM-CU F0088 were kinds of thermostable amyloltic enzymes and able to digest raw corn starch.
一株新分离的生淀粉消化酶活力较高的根霉，经形态学特征和分子系统发育分析鉴定为中国华根霉变种（Rhizopus microsporus var. chinensis）CICIM-CU F0088。该菌株从中国胶冻布丁厂的土壤中分离得到，在添加淀粉和麦麸的固态发酵条件下，产酶活力较高。薄层层析结果表明，该菌株产生两种淀粉水解酶，包括一种α-淀粉酶和两种糖化酶。电镜观察发现，两种糖化酶能消化生玉米淀粉，最适温度为 70℃。这些结果表明，中国华根霉变种 CICIM-CU F0088 分泌的淀粉水解酶是热稳定的淀粉水解酶，能消化生玉米淀粉。
