Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, USA.
Nat Chem Biol. 2010 Apr;6(4):270-2. doi: 10.1038/nchembio.316. Epub 2010 Mar 7.
The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification.
泛素生物学的快速发展需要简便的化学方法来进行蛋白质泛素化，以克服由泛素连接酶催化的酶反应所面临的低产量的常见问题。我们通过二硫键交换和内含肽化学报告了一种用于 PCNA 的单泛素化和 SUMO 化的化学方法。我们在研究跨损伤 DNA 合成时使用了化学泛素化和 SUMO 化的 PCNA，并揭示了泛素修饰的惊人的灵活性。
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