Yan Shuo, Gao Wenyuan, Lu Fuping, Zhao Runhuai
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2721-4.
To substantiate the effects of spaceflight on the glycyrrhizic acid-related gene mutation in Glycyrrhiza uralensis.
Licorice (G. uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0. 102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After returned to the earth, the satellite-flown seeds and the un-flown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on the glycyrrhizic acid-related gene mutation including ITS sequence and beta-amyrine synthase gene.
The ITS sequence of glycyrrhizic acid related gene showed no changes after spaceflight. While beta-amyrine synthase gene had some different points after spaceflight and the different points could get the expression.
The results indicated that spaceflight induce genetic variation in G. uralensis and spaceflight could also have effects on glycyrrhizic acid-related gene mutation in G. uralensis. It may need to further research how the spaceflight induced the mutation of the glycyrrhizic acid related gene. The results suggested that recoverable satellite-flown condition could bring inheritable mutagenic effects on G. uralensis seeds and maybe used as a tool for accelerating the progress in G. uralensis breeding.
证实航天飞行对乌拉尔甘草中甘草酸相关基因突变的影响。
甘草种子由一颗返回式卫星搭载18天（返回舱内平均辐射剂量为0.102 m×d⁻¹，轨道半长径350 km，重力10⁻⁶）。返回地球后，将搭载卫星的种子和未搭载的种子（地面对照）种植在试验农场的田地里。每组的叶片用于研究航天飞行对甘草酸相关基因突变的影响，包括ITS序列和β-香树脂醇合成酶基因。
甘草酸相关基因的ITS序列在航天飞行后未显示变化。而β-香树脂醇合成酶基因在航天飞行后有一些不同点，且这些不同点能够表达。
结果表明航天飞行可诱导乌拉尔甘草发生遗传变异，也可能对乌拉尔甘草中甘草酸相关基因突变产生影响。航天飞行如何诱导甘草酸相关基因突变可能需要进一步研究。结果提示返回式卫星搭载条件可对乌拉尔甘草种子产生可遗传的诱变效应，或许可作为加速乌拉尔甘草育种进程的一种手段。
