Shen Zhanyun, Liu Chunsheng, Wang Xueyong, Guo Wei, Li Beining
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(7):813-6. doi: 10.4268/cjcmm20100701.
To analyze the correlation between content of glycyrrhizic acid and the single nucleotide polymorphism of beta-amyrin synthase (bAS) in Glycyrrhiza uralensis.
glycyrrhizic acid content in 80 samples of the cultivated G. uralensis were determined by HPLC; According to the very significant level (P < 0.000 1), 80 samples in accordance with glycyrrhizic acid will be grouped by SAS 9.0; Using RT-PCR strategy to amplification the Open Reading Frame of beta-amyrin synthase with the template of total RNA extracted from roots of G. uralensis and then using DNAman to analyze the relationship between glycyrrhizic acid content and the single nucleotide polymorphism of beta-amyrin synthase (bAS).
There exited two mutation sites 94 bp and 254 bp, G/A conversion occurred at 94 bp site, which belonged to a missense mutation. G/A conversion led to the corresponding amino acid conversion (Gly --> Asp); C/T conversion occurred at 254 bp site, which belonged to a synonymous mutation. According to sequence variation, the samples were divided into four genotypes: G-T genotype, A-T genotype, G/A-C genotype and G-T genotype.
A-T genotype, G/A-C genotype and G-T genotype are correlated with the high content of glycyrrhizic acid.
分析乌拉尔甘草中甘草酸含量与β-香树脂醇合酶（bAS）单核苷酸多态性之间的相关性。
采用高效液相色谱法测定80份栽培乌拉尔甘草样品中的甘草酸含量；根据极显著水平（P<0.000 1），用SAS 9.0软件按甘草酸含量对80份样品进行分组；以乌拉尔甘草根中提取的总RNA为模板，采用RT-PCR策略扩增β-香树脂醇合酶的开放阅读框，然后用DNAman软件分析甘草酸含量与β-香树脂醇合酶（bAS）单核苷酸多态性之间的关系。
存在两个突变位点，分别位于94 bp和254 bp处，94 bp位点发生G/A转换，属于错义突变，导致相应氨基酸发生转换（甘氨酸→天冬氨酸）；254 bp位点发生C/T转换，属于同义突变。根据序列变异，将样品分为4种基因型：G-T基因型、A-T基因型、G/A-C基因型和G-T基因型。
A-T基因型、G/A-C基因型和G-T基因型与甘草酸高含量相关。
