Zhou Xiqin, Zhang Qingying, Liang Hong, Jiang Yajie, Wang Xuan, Wang Bin, Zhao Yuying
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2910-5.
To develop a HPLC-DAD method for simultaneous determination of six flavonoids in Scutellaria baicalensis, and study the effect of geographic sources, culturation, harvesting time and processing on the contents of flavonoids.
The analysis was performed on a Thermo ODS-2 Hypersil column (4.6 mm x 250 mm, 5 microm) at 26 degrees C with a gradient program of acetonitrile-0.1% H3 PO4 aqueous solution-tetrahydrofuran as the mobile phase and a Diode Array Detector as the detector with the detection wavelength at 274 nm. The flow rate was 1.0 mL x min(-1), and the injection value was 20 microL.
Six flavonoids, baicalin (1), oroxylin A-7-O-glucuronide (2), wogonoside (3), baicalein (4), wogonin (5) and oroxylin A (6), were linear over the selected range with R2 > or = 0.999 3. The average recoveries were between 96.6% -103.0%, and RSD were less then 5.0% (n = 9) for flavonoids 1-6. By analyzing the data obtained, the effect of geographic sources, culturation, harvesting time and processing on the contents of flavonoids were discussed.
This method is simple, sensitive, reliable and reproducible. It can be used for the quality control of S. baicalensis.
建立高效液相色谱-二极管阵列检测法同时测定黄芩中6种黄酮类成分的含量，并研究产地、栽培、采收时间及加工方法对黄酮类成分含量的影响。
采用Thermo ODS-2 Hypersil色谱柱（4.6 mm×250 mm，5μm），柱温26℃，以乙腈-0.1%磷酸水溶液-四氢呋喃为流动相进行梯度洗脱，二极管阵列检测器检测，检测波长为274 nm。流速为1.0 mL·min-1，进样量为20μL。
黄芩苷（1）、木犀草素-7-O-葡萄糖醛酸苷（2）、汉黄芩苷（3）、黄芩素（4）、汉黄芩素（5）和木犀草素（6）6种黄酮类成分在所考察的浓度范围内线性关系良好，R2≥0.999 3。1～6号黄酮类成分的平均回收率在96.6%～103.0%之间，相对标准偏差（RSD）均小于5.0%（n = 9）。通过对所得数据的分析，讨论了产地、栽培、采收时间及加工方法对黄酮类成分含量的影响。
该方法简便、灵敏、可靠、重现性好，可用于黄芩的质量控制。
