Kruip T A, Pieterse M C, van Beneden T H, Vos P L, Wurth Y A, Taverne M A
Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.
Vet Rec. 1991 Mar 2;128(9):208-10. doi: 10.1136/vr.128.9.208.
Eight cows were used to study the feasibility of transvaginal ultrasound-guided puncture of follicles as a method for the collection of immature oocytes for embryo production in vitro. In six trials at intervals of seven days, 104 oocytes were collected. After in vitro maturation and fertilisation the 104 oocytes were transferred to the oviducts of sheep. Six days later, 75 oocytes were recovered by flushing the oviducts. Twenty-four per cent of the recovered oocytes/embryos had developed into transferable and viable morulae and, or, blastocysts. The data show that this non-surgical and repeated collection of immature oocytes can be used successfully for the in vitro production of bovine embryos. The procedure may produce yields of embryos comparable to those obtainable by conventional superovulation procedures.
选用8头奶牛研究经阴道超声引导卵泡穿刺术作为收集未成熟卵母细胞用于体外胚胎生产方法的可行性。在间隔7天的6次试验中，共收集到104枚卵母细胞。经体外成熟和受精后，将这104枚卵母细胞移植到绵羊输卵管中。6天后，通过冲洗输卵管回收了75枚卵母细胞。回收的卵母细胞/胚胎中有24%发育成了可移植且存活的桑葚胚和/或囊胚。数据表明，这种非手术且可重复收集未成熟卵母细胞的方法可成功用于体外生产牛胚胎。该方法产生的胚胎产量可能与传统超数排卵方法相当。
