Behboodi E, Van Metre D C, BonDurant R H, Anderson G B
Department of Animal Science, School of Veterinary Medicine, University of California, Davis 95616.
J Am Vet Med Assoc. 1994 Dec 1;205(11):1588-9.
An aged Holstein cow of high genetic merit and economic value was determined to have a squamous cell carcinoma of the tarsus. Treatment was unsuccessful, as was an attempt at superovulation and embryo collection; thus, the owner elected euthanasia. On day 17 of the estrous cycle, the ovaries were removed via laparotomy and the cow was immediately euthanatized. Oocytes were aspirated from ovarian follicles and subjected to in vitro maturation and fertilization procedures. Seven morulae that developed in culture were transferred nonsurgically to 3 recipient females, resulting in birth of a full-term healthy male calf. In vitro maturation and fertilization procedures can be used to obtain offspring from valuable, terminally ill cows for which conventional embryo collection techniques have proven unsuccessful.
一头具有高遗传价值和经济价值的老龄荷斯坦奶牛被诊断患有跗关节鳞状细胞癌。治疗失败，超数排卵和胚胎采集的尝试也未成功；因此，主人选择实施安乐死。在发情周期的第17天，通过剖腹术摘除卵巢，随后立即对奶牛实施安乐死。从卵巢卵泡中吸出卵母细胞，并进行体外成熟和受精程序。培养过程中发育的7个桑葚胚被非手术移植到3头受体母牛体内，最终产下一头足月健康的雄性小牛。对于传统胚胎采集技术已证明失败的有价值的绝症母牛，体外成熟和受精程序可用于获得后代。
