应用光学相干断层成像术检测沙鼠颊囊模型中发泡剂诱导的上呼吸道黏膜损伤。
Detection of vesicant-induced upper airway mucosa damage in the hamster cheek pouch model using optical coherence tomography.
机构信息
University of California, Beckman Laser Institute and Medical Clinic, Irvine, California 92612, USA.
出版信息
J Biomed Opt. 2010 Jan-Feb;15(1):016017. doi: 10.1117/1.3309455.
Abstract
Hamster cheek pouches were exposed to 2-chloroethyl ethyl sulfide [CEES, half-mustard gas (HMG)] at a concentration of 0.4, 2.0, or 5.0 mg/ml for 1 or 5 min. Twenty-four hours post-HMG exposure, tissue damage was assessed by both stereomicrography and optical coherence tomography (OCT). Damage that was not visible on gross visual examination was apparent in the OCT images. Tissue changes were found to be dependent on both HMG concentration and exposure time. The submucosal and muscle layers of the cheek pouch tissue showed the greatest amount of structural alteration. Routine light microscope histology was performed to confirm the OCT observations.
摘要
仓鼠颊囊在浓度为 0.4、2.0 或 5.0mg/ml 的 2-氯乙基乙基硫醚（CEES，半芥子气（HMG））中暴露 1 或 5 分钟。在 HMG 暴露后 24 小时，通过立体显微镜和光学相干断层扫描（OCT）评估组织损伤。在大体视觉检查中看不到的损伤在 OCT 图像中是明显的。组织变化取决于 HMG 浓度和暴露时间。颊囊组织的粘膜下层和肌肉层显示出最大程度的结构改变。进行常规光镜组织学检查以确认 OCT 观察结果。
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