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用于快速检测具有流行病学和临床意义的结核分枝杆菌北京基因型分离株的实时聚合酶链反应检测法

Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates.

作者信息

Mokrousov Igor, Vyazovaya Anna, Zhuravlev Viacheslav, Otten Tatiana, Millet Julie, Jiao Wei-Wei, Shen A-Dong, Rastogi Nalin, Vishnevsky Boris, Narvskaya Olga

机构信息

Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia.

出版信息

J Clin Microbiol. 2014 May;52(5):1691-3. doi: 10.1128/JCM.03193-13. Epub 2014 Feb 12.

DOI:10.1128/JCM.03193-13
PMID:24523461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3993684/
Abstract

Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its global propagation) and with clinical specimens.

摘要

结核分枝杆菌北京基因型菌株正在迅速传播，通常具有高毒力且耐多药。在此，我们描述了一种通过实时PCR快速检测它们的方法，该方法靶向dnaA-dnaN基因组区域中的特定IS6110插入序列。我们使用了一个在地理和遗传上具有多样性的样本集对该方法进行评估，该样本集代表了东亚和前苏联地区，这些地区是北京基因型地方性流行和流行性的区域（即其全球传播的主要疫源地），同时还使用了临床标本进行评估。

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本文引用的文献

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Insights into the origin, emergence, and current spread of a successful Russian clone of Mycobacterium tuberculosis.深入了解成功俄罗斯分枝杆菌结核分枝杆菌克隆的起源、出现和当前传播情况。
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SITVITWEB--a publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology.SITVITWEB--一个公开的国际多标记数据库，用于研究结核分枝杆菌遗传多样性和分子流行病学。
Infect Genet Evol. 2012 Jun;12(4):755-66. doi: 10.1016/j.meegid.2012.02.004. Epub 2012 Feb 17.
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'Lethal' combination of Mycobacterium tuberculosis Beijing genotype and human CD209 -336G allele in Russian male population.俄罗斯男性人群中结核分枝杆菌北京基因型和人类 CD209-336G 等位基因的“致命”组合。
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"Pseudo-Beijing": evidence for convergent evolution in the direct repeat region of Mycobacterium tuberculosis."伪北京"：结核分枝杆菌重复序列区的趋同进化证据。
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