Jones R N, Edson D C
University of Iowa College of Medicine, Iowa City.
Arch Pathol Lab Med. 1991 May;115(5):429-36.
The ability of the College of American Pathologists Microbiology Surveys subscriber laboratories to perform antimicrobial susceptibility testing accurately has improved slightly since 1984. Currently (1989 surveys), the accuracies for disk diffusion and minimum inhibitory concentration antimicrobial susceptibility testing were 98.2% and 96.1%, respectively. Disk diffusion testing has recently (since 1986) become more popular, along with rapid automated systems, such as the AMS-Vitek System (St Louis, Mo). Rapid tests for beta-lactamase and chloramphenicol acetyltransferase have performed well. Quality control procedures have switched to a cost-effective weekly frequency pattern for nearly 70% of laboratories. Some antimicrobial susceptibility testing problems still exist among anaerobic bacterial methods, procedures for fastidious organisms (Haemophilus, Streptococcus species, Moraxella, pneumococci, gonococci), tests for oxacillin-resistant staphylococci, and the methods for use against nonenteric gram-negative or gram-positive bacilli. Antimicrobial susceptibility testing users subscribing to the College of American Pathologists surveys should strictly follow the National Committee for Clinical Laboratory Standards interpretive and quality control criteria to assure the best performance with the Clinical Laboratory Improvement Act, 1988, compliant College of American Pathologists proficiency sample program.
自1984年以来，美国病理学家学会微生物学调查的参与实验室准确进行抗菌药物敏感性试验的能力略有提高。目前（1989年调查），纸片扩散法和最低抑菌浓度抗菌药物敏感性试验的准确率分别为98.2%和96.1%。最近（自1986年以来），纸片扩散试验以及快速自动化系统，如AMS-Vitek系统（密苏里州圣路易斯）变得更受欢迎。β-内酰胺酶和氯霉素乙酰转移酶的快速检测表现良好。近70%的实验室的质量控制程序已改为具有成本效益的每周一次的频率模式。在厌氧菌检测方法、苛养菌（嗜血杆菌、链球菌属、莫拉菌属、肺炎球菌、淋球菌）检测程序、耐苯唑西林葡萄球菌检测以及针对非肠道革兰阴性或革兰阳性杆菌的检测方法中，仍存在一些抗菌药物敏感性试验问题。订阅美国病理学家学会调查的抗菌药物敏感性试验用户应严格遵循美国国家临床实验室标准委员会的解释和质量控制标准，以确保在符合1988年《临床实验室改进法案》的美国病理学家学会能力验证样本计划中获得最佳性能。
