Hu B, Jin D, Fan W X, Peng S K, Morin R J
Institute on Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing.
Artery. 1991;18(2):87-98.
Rabbit aortic endothelial cells in culture were incubated with 10 micrograms/ml of either pure cholesterol or cholestane-3 beta, 5 alpha, 6 beta-triol (triol) for 12 or 24 hours. Cells incubated with triol showed an increased rate of cell death, decreased [3H]-thymidine incorporation and decreased prostacyclin production as compared to either the control or the pure cholesterol group. These effects were more marked at 24 hours than at 12 hours. Pure cholesterol had no significant effects on any of the above parameters at either time interval. These results suggest potential atherogenic mechanisms for cholestanetriol, one of the most biologically active cholesterol oxidation products.
将培养的兔主动脉内皮细胞与10微克/毫升的纯胆固醇或胆甾烷-3β,5α,6β-三醇（三醇）一起孵育12或24小时。与对照组或纯胆固醇组相比，用三醇孵育的细胞显示出细胞死亡率增加、[3H] - 胸苷掺入减少和前列环素生成减少。这些效应在24小时时比12小时时更明显。在两个时间间隔内，纯胆固醇对上述任何参数均无显著影响。这些结果表明胆甾三醇（最具生物活性的胆固醇氧化产物之一）具有潜在的致动脉粥样硬化机制。
