Department of Orthopaedic Surgery, Navy General Hospital, Beijing, PR China.
Tissue Eng Part A. 2010 Jul;16(7):2381-9. doi: 10.1089/ten.TEA.2009.0770.
The aims of this study were to construct the composite of poly (L-lactic-co-glycolic acid) (PLGA) scaffold-nucleus pulposus (NP) cells using tissue engineering methods and to investigate the in vivo performance of the composite in a canine model. NP cells were isolated from the lumbar intervertebral discs of a beagle dog. NP cells were cultured, expanded in vitro, and seeded onto a three-dimensional porous PLGA scaffold. The composite was tested in 18 beagle dogs that were randomly divided into three groups: nucleotomy alone (A), nucleotomy with PLGA implantation (B), and nucleotomy with PLGA scaffold/NP cells composite implantation (C). X-ray and magnetic resonance imaging were performed pre- and postoperatively. Evaluation of disc height, segment stability, and biomechanics and immunohistochemical analysis were performed. Dog NP cells attached and showed proliferation activity within the PLGA scaffold in vitro and in vivo. Disc height, segmental stability, and T2-weighted signal intensity on magnetic resonance imaging scans were well preserved in group C dogs with the engineered composite. PHK-26-positive cells were found within the area of the NP 8 weeks postoperatively. The NP cell-PLGA scaffold composite can prevent or delay the degeneration process after nucleotomy in the canine model. This hybrid composite might be a promising construct for intervertebral disc regeneration.
本研究的目的是采用组织工程学方法构建聚（L-丙交酯-共-乙交酯）（PLGA）支架-髓核（NP）细胞复合材料，并在犬模型中研究其体内性能。NP 细胞从比格犬腰椎间盘分离。NP 细胞在体外培养、扩增，并接种到三维多孔 PLGA 支架上。将该复合材料在 18 只比格犬中进行测试，随机分为三组：单纯髓核切除术（A）、髓核切除术加 PLGA 植入（B）和髓核切除术加 PLGA 支架/NP 细胞复合材料植入（C）。术前和术后进行 X 线和磁共振成像检查。对椎间盘高度、节段稳定性和生物力学以及免疫组织化学分析进行评估。犬 NP 细胞在体外和体内的 PLGA 支架上附着并显示增殖活性。在接受工程化复合材料治疗的 C 组犬中，椎间盘高度、节段稳定性和磁共振成像扫描的 T2 加权信号强度得到很好的保留。术后 8 周，在 NP 区发现了 PHK-26 阳性细胞。NP 细胞-PLGA 支架复合材料可防止或延迟犬模型髓核切除术后的退变过程。这种杂交复合材料可能是椎间盘再生的一种有前途的构建体。
